Boerckel Joel D, Mason Devon E, McDermott Anna M, Alsberg Eben
Stem Cell Res Ther. 2014 Dec 29;5(6):144. doi: 10.1186/scrt534.
Microcomputed tomography (microCT) has become a standard and essential tool for quantifying structure-function relationships, disease progression, and regeneration in preclinical models and has facilitated numerous scientific and bioengineering advancements over the past 30 years. In this article, we recount the early events that led to the initial development of microCT and review microCT approaches for quantitative evaluation of bone, cartilage, and cardiovascular structures, with applications in fundamental structure-function analysis, disease, tissue engineering, and numerical modeling. Finally, we address several next-generation approaches under active investigation to improve spatial resolution, acquisition time, tissue contrast, radiation dose, and functional and molecular information.
微计算机断层扫描（microCT）已成为在临床前模型中量化结构-功能关系、疾病进展和再生的标准且必不可少的工具，并且在过去30年中推动了众多科学和生物工程的进步。在本文中，我们讲述了导致microCT最初发展的早期事件，并回顾了用于定量评估骨骼、软骨和心血管结构的microCT方法，及其在基础结构-功能分析、疾病、组织工程和数值建模中的应用。最后，我们探讨了正在积极研究的几种下一代方法，以提高空间分辨率、采集时间、组织对比度、辐射剂量以及功能和分子信息。
