大鼠主动脉对乙酰胆碱无舒张反应并不仅仅意味着缺乏功能性内皮。

The inability of the rat aorta to relax to acetylcholine does not imply lack of a functional endothelium only.

Doggrell S A

Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.

Gen Pharmacol. 1989;20(4):395-8. doi: 10.1016/0306-3623(89)90184-5.

Abstract

The inability of blood vessels to relax to acetylcholine is often used as the sole test of endothelial removal. 2. Following blotting of the intimal surface of the rat aorta with filter paper, the tissue did not relax to acetylcholine and the contractions to alpha 2-adrenoceptor agonists and to the lower concentrations of noradrenaline, adrenaline, phenylephrine and KCl were enhanced. 3. However the responses to the higher concentrations of noradrenaline, adrenaline, phenylephrine and KCl were reduced. 4. These results show that blotting with filter paper destroys the endothelium and some smooth muscle.

摘要

血管对乙酰胆碱舒张反应的丧失常被用作评估内皮细胞去除情况的唯一检测方法。2. 用滤纸擦拭大鼠主动脉内膜表面后，该组织对乙酰胆碱不再舒张，对α2 -肾上腺素能受体激动剂以及较低浓度的去甲肾上腺素、肾上腺素、苯肾上腺素和氯化钾的收缩反应增强。3. 然而，对较高浓度的去甲肾上腺素、肾上腺素、苯肾上腺素和氯化钾的反应减弱。4. 这些结果表明，用滤纸擦拭会破坏内皮细胞和部分平滑肌。