Gao Shenyang, Li Dandan, Zha Enhui, Zhou Tiezhong, Wang Shen, Yue Xiqing
Department of Food Science, Shenyang Agricultral University, Shenyang 110161, China.
Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.
Int J Environ Res Public Health. 2015 Feb 11;12(2):2026-36. doi: 10.3390/ijerph120202026.
Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks.
samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis.
the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish. HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group.
13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish. Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas.
戊型肝炎病毒（HEV）已被确认为一种分布于全球的人畜共患病毒。中国对水环境中的HEV污染情况尚未进行充分研究。本研究的目的是评估中国沿海某地区贝类中的HEV污染情况。这种污染对于评估公共卫生风险具有重要意义。
从渤海湾周边13个河口潮滩采集了三种贝类样本，采用自行设计的巢式逆转录聚合酶链反应（RT-PCR）检测方法对HEV RNA进行筛查。对检测出的HEV阳性样本进一步通过基因克隆和测序分析进行验证。
贝类中HEV的总体阳性检出率约为每千克17.5%。HEV在近江牡蛎中更为常见（28.2%），其次是泥蚶（14.3%）和菲律宾蛤仔（11.5%）。对检测出的13株HEV毒株进行系统发育分析表明，基因片段分为两个已知的4型亚型（4b/4d）组和另一个未知组。
在渤海湾沿岸受污染的贝类中发现了13种不同的4型HEV亚型。研究结果表明，渤海地区的水域使用者和贝类消费者可能存在健康风险。需要进一步研究来评估这些环境中HEV的来源和传染性，并评估其他贝类捕捞区域。
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