Pitkänen O M, Hallman M, Andersson S M
Children's Hospital, Helsinki University Central Hospital, Finland.
Lipids. 1989 Feb;24(2):157-9. doi: 10.1007/BF02535255.
It has been proposed that ethane and pentane reflect free oxygen radical-induced lipid peroxidation. However, methodological difficulties limit the use of these gases for assessment of free oxygen radical activity. In the present report we describe an improved method for the accurate analysis of picomole quantities (greater than or equal to 1 pmol) of ethane and pentane. They are first quantitatively trapped into an adsorbent and then heat-desorbed directly into a capillary column for gas chromatographic quantitation. During oxidation of linolenic (n-3) and linoleic (n-6) acid, ethane and pentane were formed, respectively. Nonstimulated granulocytes formed pentane. Upon addition of phorbol 13-myristate 12-acetate, the generation of pentane was increased by 540%. Addition of superoxide dismutase plus catalase inhibited lipid peroxidation in both a cell-free system and in isolated cells. The present method is useful in the evaluation of free oxygen radical induced damage.
有人提出乙烷和戊烷反映了自由氧自由基诱导的脂质过氧化作用。然而，方法学上的困难限制了这些气体用于评估自由氧自由基活性。在本报告中，我们描述了一种改进的方法，用于准确分析皮摩尔量（大于或等于1皮摩尔）的乙烷和戊烷。它们首先被定量捕获到一种吸附剂中，然后直接热解吸到毛细管柱中进行气相色谱定量分析。在亚麻酸（n-3）和亚油酸（n-6）氧化过程中，分别生成了乙烷和戊烷。未受刺激的粒细胞生成戊烷。加入佛波酯12-肉豆蔻酸酯13-乙酸酯后，戊烷的生成增加了540%。加入超氧化物歧化酶和过氧化氢酶可抑制无细胞系统和分离细胞中的脂质过氧化作用。本方法有助于评估自由氧自由基诱导的损伤。
