Bertotto A, Crupi S, Arcangeli C, Gerli R, Marinelli I, Velardi A, Vaccaro R
Department of Paediatrics, Perugia University Medical School, Italy.
Scand J Immunol. 1989 Jul;30(1):39-43. doi: 10.1111/j.1365-3083.1989.tb01186.x.
Peripheral blood mononuclear cells from 10 subjects with cytogenetically documented Down's syndrome (DS) and from 10 age- and sex-matched healthy controls were assayed for their ability to proliferate in response to phytohaemagglutinin, anti-CD3 (OKT3), or anti-CD2 (T11(2) plus T11(3] monoclonal antibodies. Interleukin 2 (IL-2) receptor expression and IL-2 production in mitogen-pulsed lymphocyte cultures was also investigated in parallel. DS cells responded poorly to all the blastogenic stimuli used in this study. Under certain experimental conditions (anti-CD3 or anti-CD2 antibody stimulation), the patients' lymphocytes expressed low levels of IL-2 surface receptors and failed to produce normal amounts of this lymphokine. Studies are currently in progress in our laboratories to determine whether these defects are due to an impairment of the early signalling events surrounding the complexing of CD3, CD2, or lectin receptors to their respective ligands.
对10名经细胞遗传学证实患有唐氏综合征（DS）的受试者以及10名年龄和性别匹配的健康对照者的外周血单个核细胞进行检测，以评估它们对植物血凝素、抗CD3（OKT3）或抗CD2（T11(2)加T11(3)单克隆抗体）的增殖反应能力。同时，还平行研究了丝裂原刺激的淋巴细胞培养物中白细胞介素2（IL-2）受体的表达和IL-2的产生。DS细胞对本研究中使用的所有致有丝分裂刺激反应不佳。在某些实验条件下（抗CD3或抗CD2抗体刺激），患者的淋巴细胞表达低水平的IL-2表面受体，并且不能产生正常量的这种淋巴因子。我们实验室目前正在进行研究，以确定这些缺陷是否是由于围绕CD3、CD2或凝集素受体与其各自配体结合的早期信号事件受损所致。
