Matsumoto K, Yoshikai Y, Matsuzaki G, Asano T, Nomoto K
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Eur J Immunol. 1989 Jul;19(7):1203-7. doi: 10.1002/eji.1830190708.
Sequential appearance of T cell subpopulations occurs in the thymus of irradiated AKR (H-2k, Thy-1.1) mice at an early stage after transplantation with bone marrow cells of C3H/HeN (H-2k, Thy-1.2) mice. The donor-derived thymocytes were first detected on day 8 after bone marrow reconstitution. Although most of the thymocytes were CD4-CD8- cells, an appreciable level of CD4+CD8- cells was detected in the thymus at this stage. The early appearing CD4+CD8- cells were a novel subset of thymocytes that were J11d+CD3-. From day 10 to day 21 the proportion of CD4+CD8-CD3-J11d+ cells decreased while the proportion of CD4+CD8+ cells and CD4+CD8-CD3+J11d- cells increased. The CD4+CD8-CD3- cells seem to diversify to form CD4+CD8+ thymocytes after short-term culture in vitro. These results suggested the existence of a differential pathway from CD4-CD8- cells to CD4+CD8+ cells via CD4+CD8- cells in thymus.
在用C3H/HeN（H-2k，Thy-1.2）小鼠的骨髓细胞移植后的早期阶段，照射过的AKR（H-2k，Thy-1.1）小鼠的胸腺中会出现T细胞亚群的顺序性出现。在骨髓重建后的第8天首次检测到供体来源的胸腺细胞。虽然大多数胸腺细胞是CD4-CD8-细胞，但在此阶段胸腺中检测到了相当数量的CD4+CD8-细胞。早期出现的CD4+CD8-细胞是胸腺细胞的一个新亚群，即J11d+CD3-。从第10天到第21天，CD4+CD8-CD3-J11d+细胞的比例下降，而CD4+CD8+细胞和CD4+CD8-CD3+J11d-细胞的比例增加。CD4+CD8-CD3-细胞在体外短期培养后似乎会分化形成CD4+CD8+胸腺细胞。这些结果表明在胸腺中存在一条从CD4-CD8-细胞通过CD4+CD8-细胞到CD4+CD8+细胞的差异途径。
