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Differentiation of CD3-4-8- human fetal thymocytes in vivo: characterization of a CD3-4+8- intermediate.体内CD3 - 4 - 8 - 人胎儿胸腺细胞的分化：CD3 - 4 + 8 - 中间阶段的特征
J Exp Med. 1993 Jul 1;178(1):265-77. doi: 10.1084/jem.178.1.265.
2
Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development.人类胸腺中CD3+CD4+CD8+细胞的前体由CD34的表达来定义。人类胸腺发育早期事件的描述。
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3
Programmed differentiation of murine thymocytes during fetal thymus organ culture.胎儿胸腺器官培养过程中小鼠胸腺细胞的程序性分化。
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The kinetics of T cell antigen receptor expression by subgroups of CD4+8+ thymocytes: delineation of CD4+8+3(2+) thymocytes as post-selection intermediates leading to mature T cells.CD4+8+胸腺细胞亚群T细胞抗原受体表达的动力学：将CD4+8+3(2+)胸腺细胞描绘为通向成熟T细胞的选择后中间体。
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Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function.人类CD4+ T细胞近期胸腺迁出细胞通过蛋白酪氨酸激酶7得以识别，且免疫功能降低。
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Thymus cell migration. Quantitative aspects of cellular traffic from the thymus to the periphery in mice.胸腺细胞迁移。小鼠体内细胞从胸腺向外周迁移的定量研究。
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T cell tolerance by clonal elimination in the thymus.胸腺中通过克隆清除实现的T细胞耐受性。
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Limiting dilution analysis of the stem cells for T cell lineage.T细胞谱系干细胞的有限稀释分析。
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体内CD3 - 4 - 8 - 人胎儿胸腺细胞的分化：CD3 - 4 + 8 - 中间阶段的特征

Differentiation of CD3-4-8- human fetal thymocytes in vivo: characterization of a CD3-4+8- intermediate.

作者信息

Kraft D L, Weissman I L, Waller E K

机构信息

Cancer Biology Research Laboratory, Stanford University School of Medicine, California 94305.

出版信息

J Exp Med. 1993 Jul 1;178(1):265-77. doi: 10.1084/jem.178.1.265.

DOI:10.1084/jem.178.1.265
PMID:8315382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2191096/
Abstract

Human thymocyte differentiation was examined by injecting fetal thymic progenitor populations into human thymic xenografts in SCID-hu mice. Thymic progenitors were fluorescently labeled with the lipophilic dye PKH2. The phenotypes of their progeny could be identified by flow cytometric analysis of cells with a very high fluorescent PKH2 signal. Intrathymic injection of purified triple negative (TN) CD3-4-8- thymocytes resulted in the sequential appearance of CD3-4+8-, CD3-4+8+, and CD3+4+8+ cells, with the subsequent appearance of small numbers of phenotypically mature CD3+4+8- and CD3+4-8+ cells over a 4-d period. Sorted CD3-4+8- thymocytes injected intrathymically rapidly differentiated to CD4+8+ cells. CD4+8+ fetal thymocytes in cell cycle differentiated into phenotypically mature CD3+4+8- and CD3+4-8+ populations, whereas nondividing CD4+8+ cells failed to differentiate after intrathymic transfer. The number of cell divisions that occurred between the injection of TN thymocytes and their progeny at different time points was estimated based on the decrease in the intensity of the PKH2 label. The average length of the cell cycle for the TN population was calculated to be 24 h. The SCID-hu model thus provides a useful tool for studying the kinetics of cell division and differentiation of human thymocytes in vivo.

摘要

通过将胎儿胸腺祖细胞群体注射到SCID-hu小鼠的人胸腺异种移植物中，研究了人胸腺细胞的分化。胸腺祖细胞用亲脂性染料PKH2进行荧光标记。通过对具有非常高荧光PKH2信号的细胞进行流式细胞术分析，可以鉴定其后代的表型。胸腺内注射纯化的三阴性（TN）CD3-4-8-胸腺细胞导致CD3-4+8-、CD3-4+8+和CD3+4+8+细胞依次出现，随后在4天内出现少量表型成熟的CD3+4+8-和CD3+4-8+细胞。胸腺内注射分选的CD3-4+8-胸腺细胞迅速分化为CD4+8+细胞。处于细胞周期的CD4+8+胎儿胸腺细胞分化为表型成熟的CD3+4+8-和CD3+4-8+群体，而未分裂的CD4+8+细胞在胸腺内转移后未能分化。根据PKH2标记强度的降低，估计了在不同时间点注射TN胸腺细胞与其后代之间发生的细胞分裂次数。计算得出TN群体的细胞周期平均长度为24小时。因此，SCID-hu模型为研究体内人胸腺细胞的细胞分裂和分化动力学提供了一个有用的工具。