Production of discrete high specific activity DNA probes using the polymerase chain reaction.
作者信息
Jansen R, Ledley F D
机构信息
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
出版信息
Gene Anal Tech. 1989 Jul-Aug;6(4):79-83. doi: 10.1016/0735-0651(89)90020-4.
PMID:2569437
Abstract
Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 x 10(9) cpm/microgram) is comparable to probes produced by other methods.
摘要
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