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本文引用的文献

1
Detection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers.通过在培养基或反应缓冲液中添加碳酸氢盐，可以提高诊断实验室中对产OXA - 48型碳青霉烯酶肠杆菌科细菌的检测能力。
Folia Microbiol (Praha). 2015 Mar;60(2):119-29. doi: 10.1007/s12223-014-0349-8. Epub 2014 Sep 29.
2
High prevalence of ST131 among CTX-M-producing Escherichia coli from community-acquired infections, in the Czech Republic.在捷克共和国社区获得性感染的产CTX-M型大肠杆菌中，ST131的高流行率。
Microb Drug Resist. 2015 Feb;21(1):74-84. doi: 10.1089/mdr.2014.0070. Epub 2014 Sep 4.
3
Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test.采用基质辅助激光解吸电离飞行时间质谱法和 Carba NP 试验对产碳青霉烯酶肠杆菌科的表型检测。
J Clin Microbiol. 2014 Nov;52(11):4075-7. doi: 10.1128/JCM.02121-14. Epub 2014 Sep 3.
4
Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories.肠杆菌科碳青霉烯酶的检测：诊断微生物实验室面临的挑战。
Clin Microbiol Infect. 2014 Sep;20(9):839-53. doi: 10.1111/1469-0691.12678. Epub 2014 Jun 14.
5
CarbAcineto NP test for rapid detection of carbapenemase-producing Acinetobacter spp.用于快速检测产碳青霉烯酶不动杆菌属的CarbAcineto NP试验
J Clin Microbiol. 2014 Jul;52(7):2359-64. doi: 10.1128/JCM.00594-14. Epub 2014 Apr 23.
6
Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test.使用改良版Carba NP试验检测产碳青霉烯酶肠杆菌科细菌时，分离培养基的影响
J Med Microbiol. 2014 May;63(Pt 5):772-776. doi: 10.1099/jmm.0.071340-0. Epub 2014 Mar 3.
7
An ongoing national intervention to contain the spread of carbapenem-resistant enterobacteriaceae.一项正在进行的全国性干预措施，旨在遏制碳青霉烯类耐药肠杆菌科的传播。
Clin Infect Dis. 2014 Mar;58(5):697-703. doi: 10.1093/cid/cit795. Epub 2013 Dec 4.
8
Carbapenemase-producing Pseudomonas aeruginosa from central Greece: molecular epidemiology and genetic analysis of class I integrons.来自希腊中部的产碳青霉烯酶铜绿假单胞菌：I类整合子的分子流行病学和基因分析
BMC Infect Dis. 2013 Oct 29;13:505. doi: 10.1186/1471-2334-13-505.
9
Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa.碳青霉烯酶快速检测试验（Carba NP 试验）用于检测产碳青霉烯酶肠杆菌科细菌和铜绿假单胞菌的评价。
Antimicrob Agents Chemother. 2013 Sep;57(9):4578-80. doi: 10.1128/AAC.00878-13. Epub 2013 Jul 1.
10
Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Enterobacter cloacae from a Czech patient previously hospitalized in Sri Lanka.从一名曾在斯里兰卡住院的捷克患者身上鉴定出一株产新德里金属β-内酰胺酶-4（NDM-4）的阴沟肠杆菌。
Folia Microbiol (Praha). 2013 Nov;58(6):547-9. doi: 10.1007/s12223-013-0247-5. Epub 2013 Apr 2.

采用NH₄HCO₃的基质辅助激光解吸电离飞行时间质谱美罗培南水解试验，一种直接检测碳青霉烯酶活性的可靠工具。

Matrix-assisted laser desorption ionization-time of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity.

作者信息

Papagiannitsis Costas C, Študentová Vendula, Izdebski Radoslaw, Oikonomou Olga, Pfeifer Yvonne, Petinaki Efthimia, Hrabák Jaroslav

机构信息

Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic

Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.

出版信息

J Clin Microbiol. 2015 May;53(5):1731-5. doi: 10.1128/JCM.03094-14. Epub 2015 Feb 18.

DOI:10.1128/JCM.03094-14
PMID:25694522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4400744/
Abstract

A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.

摘要

将基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）美罗培南水解试验与Carba NP试验进行比较，结果表明两种方法的灵敏度均较低（约76%），主要原因是OXA-48型产酶菌产生的假阴性结果。在MALDI-TOF MS试验的反应缓冲液中添加NH4HCO3可显著提高其灵敏度（98%）。使用MBT STAR-BL软件对MALDI-TOF MS试验进行自动解读，结果与手动分析结果基本一致。对于Carba NP试验，分光光度分析又发现了6株碳青霉烯酶产酶菌。