采用NH₄HCO₃的基质辅助激光解吸电离飞行时间质谱美罗培南水解试验，一种直接检测碳青霉烯酶活性的可靠工具。
Matrix-assisted laser desorption ionization-time of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity.
作者信息
Papagiannitsis Costas C, Študentová Vendula, Izdebski Radoslaw, Oikonomou Olga, Pfeifer Yvonne, Petinaki Efthimia, Hrabák Jaroslav
机构信息
Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic
Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.
出版信息
J Clin Microbiol. 2015 May;53(5):1731-5. doi: 10.1128/JCM.03094-14. Epub 2015 Feb 18.
Abstract
A comparison of a matrix-assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to the false-negative results obtained with OXA-48-type producers. The addition of NH4HCO3 to the reaction buffer for the MALDI-TOF MS assay dramatically improved its sensitivity (98%). Automatic interpretation of the MALDI-TOF MS assay, using the MBT STAR-BL software, generally agreed with the results obtained after manual analysis. For the Carba NP test, spectrophotometric analysis found six additional carbapenemase producers.
摘要
将基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）美罗培南水解试验与Carba NP试验进行比较，结果表明两种方法的灵敏度均较低（约76%），主要原因是OXA-48型产酶菌产生的假阴性结果。在MALDI-TOF MS试验的反应缓冲液中添加NH4HCO3可显著提高其灵敏度（98%）。使用MBT STAR-BL软件对MALDI-TOF MS试验进行自动解读，结果与手动分析结果基本一致。对于Carba NP试验，分光光度分析又发现了6株碳青霉烯酶产酶菌。
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