Zhang Yang, Zhang Chao, Zhong Xiewei, Zhu Dan
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China ; Key Laboratory of Biomedical Photonics of Ministry of Education, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Quant Imaging Med Surg. 2015 Feb;5(1):136-42. doi: 10.3978/j.issn.2223-4292.2014.12.02.
In vivo cortex optical imaging methods for visualization of both structural and functional architecture with high spatial-temporal resolution have shown tremendous advantages in the studies on neurons, glia and microvasculature. To overcome the strong scattering of skull above the cortex, several chronic cranial windows were proposed through craniotomy, but there are some problems. Here, an innovative skull optical clearing solution (SOCS) has been invented to make the skull transparent within 25 min, but SOCS-induced optical clearing efficacy of skull is to be evaluated.
Based on the measurements of divergence of beam spot, collimated transmittance of skull, the efficiency of skull optical clearing has been further evaluated quantitatively by comparing with the Monte Carlo simulation.
The results show that the light beam bandwidth is 5.2±0.3 mm through the initial skull, and reduces to 2.0±0.2 mm trough the treated skull with SOCS; and the calculated scattering coefficient almost decreases to one third after the treatment.
The quantitative evaluation of SOCS-induced optical clearing efficacy of skull provides an important reference for performing transcranial cortical optical imaging or operation based on skull optical clearing technique.
用于以高时空分辨率可视化结构和功能结构的体内皮层光学成像方法在神经元、神经胶质和微脉管系统的研究中显示出巨大优势。为了克服皮层上方颅骨的强散射，通过开颅手术提出了几种慢性颅骨视窗，但存在一些问题。在此，已发明一种创新的颅骨光学透明溶液（SOCS），可在25分钟内使颅骨透明，但SOCS诱导的颅骨光学透明效果有待评估。
基于束斑发散度、颅骨准直透过率的测量，通过与蒙特卡罗模拟比较，进一步定量评估颅骨光学透明效率。
结果表明，光束带宽通过初始颅骨时为5.2±0.3毫米，通过用SOCS处理的颅骨时降至2.0±0.2毫米；处理后计算的散射系数几乎降至三分之一。
SOCS诱导的颅骨光学透明效果的定量评估为基于颅骨光学透明技术进行经颅皮层光学成像或手术提供了重要参考。
