Parazzi Valentina, Lavazza Cristiana, Boldrin Valentina, Montelatici Elisa, Pallotti Francesco, Marconi Maurizio, Lazzari Lorenza
Cell Factory, Unit for Cellular Therapy and Cryobiology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italy.
Cell Transplant. 2015;24(12):2573-84. doi: 10.3727/096368915X687471. Epub 2015 Feb 18.
Platelet gel derived from peripheral blood is widely applied in many clinical fields of surgery as biomaterial containing growth factors with high proliferative properties. In 2010, we studied and patented a platelet gel derived from cord blood. In this study, due to the crucial role of the factors released by the platelet gel, we first extended the characterization of its releasate. Using a wide proteomic array and splitting the two components of the releasate, that is, platelets and plasma, we have been able to study their growth factor content. Interestingly, we discovered high levels of hormones and molecules able to support tissue growth in the cord blood platelet gel releasate and, in addition, higher concentrations of several angiogenic factors if compared with the peripheral blood counterpart. On the contrary, the latter was much richer in inflammatory factors. The second aim of our work was to study the effects on cell culture, immunophenotype, and function of mesenchymal stem cells exposed to these two platelet gel releasates as substitute for the animal serum. Since our findings nicely show that the use of the peripheral versus the cord blood platelet gel releasate can differently influence the mesenchymal stem cell commitment, we can suggest that in addition to its peculiar angiogenic properties cord blood platelet gel releasate shows excellent proliferative properties as cell culture supplement.
源自外周血的血小板凝胶作为一种含有具有高增殖特性生长因子的生物材料，在外科手术的许多临床领域中得到广泛应用。2010年，我们研究了一种源自脐带血的血小板凝胶并为其申请了专利。在本研究中，鉴于血小板凝胶释放因子的关键作用，我们首先扩展了对其释放物的特性描述。通过使用广泛的蛋白质组学阵列并将释放物的两个成分，即血小板和血浆分开，我们得以研究它们的生长因子含量。有趣的是，我们在脐带血血小板凝胶释放物中发现了高水平的能够支持组织生长的激素和分子，此外，与外周血对应物相比，几种血管生成因子的浓度更高。相反，外周血血小板凝胶释放物中富含更多的炎症因子。我们工作的第二个目的是研究这两种血小板凝胶释放物作为动物血清替代品时，对间充质干细胞的细胞培养、免疫表型和功能的影响。由于我们的研究结果很好地表明，使用外周血与脐带血血小板凝胶释放物会对间充质干细胞的分化产生不同影响，我们可以认为，除了其独特的血管生成特性外，脐带血血小板凝胶释放物作为细胞培养补充剂还具有出色的增殖特性。
