Zaĭkova Iu Ia, Kulichkova V A, Mitenkova K A, Tsimokha A S
Tsitologiia. 2014;56(6):427-32.
In this paper, we present a comparative analysis of different methods of purification of proteasomes from the culture medium in which proerithroleukemia human K562 cells were grown. The results obtained allowed us to purify proteasomes from samples of conditioned cell culture medium and control the quality of the proteasome preparations at all stages of their separation. Extracellular proteasomes purified via different approaches possess all the three types of peptidase activity described for intracellular counterparts.
在本文中，我们对从培养过人类红白血病K562细胞的培养基中纯化蛋白酶体的不同方法进行了比较分析。所得结果使我们能够从条件性细胞培养基样品中纯化蛋白酶体，并在其分离的各个阶段控制蛋白酶体制剂的质量。通过不同方法纯化的细胞外蛋白酶体具有细胞内对应物所描述的所有三种肽酶活性类型。
