Quinlan M P
Cold Spring Harbor Laboratory, New York 11724.
Oncogene. 1989 Aug;4(8):1051-5.
The 12S gene of the E1A region of adenovirus induces the production of a growth factor that is necessary for the immortalization of primary epithelial cells. This growth factor is able to replace the strict serum requirement of F9 cells, a teratocarcinoma-derived embryonal carcinoma cell line. Furthermore, the growth factor induces a morphological alteration in the F9 cells such that they resemble visceral endoderm cells and express the surface antigens, SSEA-1 and SSEA-3, characteristic of these differentiated cells.
腺病毒E1A区域的12S基因诱导产生一种生长因子，该生长因子是原代上皮细胞永生化所必需的。这种生长因子能够替代F9细胞（一种源自畸胎癌的胚胎癌细胞系）对血清的严格需求。此外，该生长因子会诱导F9细胞发生形态改变，使其类似于脏内胚层细胞，并表达这些分化细胞特有的表面抗原SSEA-1和SSEA-3。
