单子叶 3 基因，在水稻中与 WUSCHEL 同源，对于分蘖芽的形成是必需的。
MONOCULM 3, an ortholog of WUSCHEL in rice, is required for tiller bud formation.
机构信息
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
J Genet Genomics. 2015 Feb 20;42(2):71-8. doi: 10.1016/j.jgg.2014.12.005. Epub 2015 Jan 12.
WUSCHEL (WUS) plays an essential role for the maintenance of meristem activity in dicots, but its function is still elusive in monocots. We isolated a new monoculm mutant, monoculm 3 (moc3), in which a point mutation causes the premature termination of rice O. sativa WUS (OsWUS). Morphological observation revealed that the formation of tiller buds was disrupted in moc3. MOC3 was localized in the nuclear and could interact with TOPLESS-RELATED PROTEINS (TPRs). The expression of MOC3 was induced by cytokinins and defection of MOC3 affected the expression of several two-component cytokinin response regulators, OsRRs and ORRs. Our results suggest that MOC3 is required for the formation of axillary buds and has a complex relationship with cytokinins.
WUSCHEL（WUS）在双子叶植物的分生组织活性维持中起着至关重要的作用，但它在单子叶植物中的功能仍不清楚。我们在水稻中分离到一个新的单茎突变体 monoculm 3（moc3），其点突变导致水稻 O. sativa WUS（OsWUS）过早终止。形态观察显示，moc3 中分蘖芽的形成受到干扰。MOC3 定位于核内，并能与 TOPLESS-RELATED PROTEINS（TPRs）相互作用。MOC3 的表达受细胞分裂素诱导，MOC3 的缺失会影响几个双组分细胞分裂素反应调节剂 OsRRs 和 ORRs 的表达。我们的结果表明，MOC3 是腋芽形成所必需的，并且与细胞分裂素有复杂的关系。
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