Li Zenghe, Guo Song, Lu Chao
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Analyst. 2015 Apr 21;140(8):2719-25. doi: 10.1039/c5an00017c.
In this study, S(2-) ions were found to enhance the fluorescence of cysteine-capped Cu nanoclusters (Cu NCs). High resolution transmission electron microscopy (HRTEM), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy-energy dispersive X-ray (SEM-EDX) measurements, zeta potential and X-ray photoelectron spectroscopy (XPS) measurements demonstrated that the S(2-) ion-induced aggregation of the Cu NCs contributed to the fluorescence enhancement of the dispersed Cu NCs. Based on these findings, a highly selective fluorescent probe was developed for the determination of H2S using the S(2-) ion-enhanced fluorescence of the as-prepared Cu NCs. The relative fluorescence intensity was proportional to the concentration of S(2-) in the range from 0.2 to 50 μM. The detection limit (S/N = 3) was 42 nM. The proposed method has been successfully applied to determine H2S produced from toys called "Fart Bomb" with recoveries of 97.6-101.8%. The results of the proposed method were in good agreement with those determined by a standard methylene blue method. This work is not only of importance for a better understanding of the aggregation induced emission (AIE) properties of the Cu NCs but also of great potential to find extensive biological applications for H2S.
在本研究中，发现S(2-)离子可增强半胱氨酸封端的铜纳米簇（Cu NCs）的荧光。高分辨率透射电子显微镜（HRTEM）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜-能量色散X射线（SEM-EDX）测量、zeta电位和X射线光电子能谱（XPS）测量表明，S(2-)离子诱导的Cu NCs聚集有助于分散的Cu NCs的荧光增强。基于这些发现，利用所制备的Cu NCs的S(2-)离子增强荧光开发了一种用于测定H2S的高选择性荧光探针。相对荧光强度与0.2至50 μM范围内的S(2-)浓度成正比。检测限（S/N = 3）为42 nM。所提出的方法已成功应用于测定名为“放屁炸弹”的玩具产生的H2S，回收率为97.6 - 101.8%。所提出方法的结果与标准亚甲基蓝法测定的结果高度一致。这项工作不仅对于更好地理解Cu NCs的聚集诱导发光（AIE）特性具有重要意义，而且对于发现H2S广泛的生物学应用具有巨大潜力。
