Wang Qiaoling, Chen Kaimin, Qu Yi, Li Kai, Zhang Ying, Fu Enyu
College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
Polymers (Basel). 2020 Mar 5;12(3):577. doi: 10.3390/polym12030577.
Currently, it is an ongoing challenge to develop fluorescent nanosphere detectors that are uniform, non-toxic, stable and bearing a large number of functional groups on the surface for further applications in a variety of fields. Here, we have synthesized hairy nanospheres (HNs) with different particle sizes and a content range of carboxyl groups from 4 mmol/g to 9 mmol/g. Based on this, hairy fluorescent nanospheres (HFNs) were prepared by the traditional coupling method (TCM) or adsorption-induced coupling method (ACM). By comparison, it was found that high brightness HFNs are fabricated based on HNs with poly (acrylic acid) brushes on the surface via ACM. The fluorescence intensity of hairy fluorescent nanospheres could be controlled by tuning the content of 5-aminofluorescein (5-AF) or the carboxyl groups of HNs easily. The carboxyl content of the HFNs could be as high as 8 mmol/g for further applications. The obtained HFNs are used for the detection of heavy metal ions in environmental pollution. Among various other metal ions, the response to Cu (II) is more obvious. We demonstrated that HFNs can serve as a selective probe and for the separation and determination of Cu(II) ions with a linear range of 0-0.5 μM and a low detection limit of 64 nM.
目前，开发出均匀、无毒、稳定且表面带有大量官能团以便在各种领域进一步应用的荧光纳米球探测器仍是一项持续的挑战。在此，我们合成了具有不同粒径且羧基含量范围为4 mmol/g至9 mmol/g的毛状纳米球（HNs）。基于此，通过传统偶联法（TCM）或吸附诱导偶联法（ACM）制备了毛状荧光纳米球（HFNs）。通过比较发现，基于表面带有聚（丙烯酸）刷的HNs，通过ACM制备出了高亮度的HFNs。毛状荧光纳米球的荧光强度可以通过轻松调节5-氨基荧光素（5-AF）的含量或HNs的羧基来控制。HFNs的羧基含量可高达8 mmol/g以供进一步应用。所获得的HFNs用于检测环境污染中的重金属离子。在各种其他金属离子中，对Cu（II）的响应更为明显。我们证明HFNs可作为一种选择性探针，用于分离和测定Cu（II）离子，线性范围为0 - 0.5 μM，检测限低至64 nM。
