谷胱甘肽S-转移酶M1、谷胱甘肽S-转移酶T1和醌氧化还原酶1基因多态性与宫颈癌发生
GSTM1, GSTT1, and NQO1 polymorphisms in cervical carcinogenesis.
作者信息
Nunobiki Osamu, Ueda Masatsugu, Akise Hikari, Izuma Shinji, Torii Kiyo, Okamoto Yoshiaki, Tanaka Ichiro, Noda Sadamu, Akashi Kyoko, Higashida Taro
机构信息
Department of Medical Technology, Kobe Tokiwa University, 6-2 2 chome, Ohtanicho, Nagata-ku, Kobe, Hyogo, 6530838, Japan,
出版信息
Hum Cell. 2015 Jul;28(3):109-13. doi: 10.1007/s13577-015-0111-9. Epub 2015 Feb 20.
The aim of the study is to investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1, and NQO1 c.609C>T (rs1800566) genetic polymorphisms in cervical carcinogenesis. GSTM1, GSTT1, and NQO1 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 192 cervical smear in exfoliated cervical cell samples using polymerase chain reaction (PCR) system and real-time polymerase chain reaction (PCR) system. The 19 patients with high-grade squamous intraepithelial lesion had statistically higher frequency of null GSTT1 genotype than 9 with low-grade squamous intraepithelial lesion (LSIL) among the 67 patients with high-risk HPV (P = 0.024). The 24 patients with HSIL had also statistically higher frequency of NQO1 (CT+TT) genotype than 14 with LSIL among the 67 patients with high-risk HPV (P = 0.024). GSTT1 null and NQO1 genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population.
本研究的目的是探讨谷胱甘肽 - S - 转移酶GSTM1、GSTT1和NQO1基因c.609C>T（rs1800566）多态性在宫颈癌发生中的临床意义。采用聚合酶链反应（PCR）系统和实时聚合酶链反应（PCR）系统，对192份宫颈脱落细胞样本的宫颈涂片进行了GSTM1、GSTT1和NQO1多态性以及人乳头瘤病毒（HPV）类型的检测。在67例高危型HPV患者中，19例高级别鳞状上皮内病变患者的GSTT1基因缺失型频率在统计学上高于9例低级别鳞状上皮内病变（LSIL）患者（P = 0.024）。同样，在67例高危型HPV患者中，24例高级别鳞状上皮内病变（HSIL）患者的NQO1（CT + TT）基因型频率在统计学上也高于14例低级别鳞状上皮内病变患者（P = 0.024）。在日本人群中，宫颈细胞样本中的GSTT1基因缺失型和NQO1基因型可能与更严重的宫颈癌前病变有关。
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