Sen Halil Murat, Ozkan Adile, Guven Mustafa, Akman Tarık, Aras Adem Bozkurt, Sehitoglu Ibrahim, Alacam Hasan, Silan Coskun, Cosar Murat, Ozisik Karaman Handan Isın
Department of Neurology, Faculty of Medicine, Canakkale Onsekiz Mart University, Terzioğlu Kampüsü, Canakkale, Turkey,
Inflammation. 2015 Aug;38(4):1624-30. doi: 10.1007/s10753-015-0138-9.
Many studies of brain ischemia have shown the role played by massive ischemia-induced production of reactive oxygen species, the main mechanism of neuronal death. However, currently, there is no treatment choice to prevent cell death triggered by reactive oxygen species. In our study, we researched the effects of tannic acid, an antioxidant, on the ischemic tissue of rats with induced middle cerebral artery occlusion. The animals were divided into three groups of eight animals. The sham group were only administered 10 % ethanol intraperitoneally, the second group had middle cerebral artery occlusion induced and were given 10 % ethanol intraperitoneally, while the third group had middle cerebral artery occlusion with 10 mg/kg dose tannic acid dissolved in 10 % ethanol administered within half an hour intraperitoneally. The rats were sacrificed 24 h later, and brain tissue was examined biochemically and histopathologically. Biochemical evaluation of brain tissue found that comparing the ischemic group with no treatment with the tannic acid-treated ischemia group; the superoxide dismutase (SOD) levels were higher, malondialdehyde (MDA) levels were lower, and nuclear respiratory factor-1 (NRF-1) was higher in the tannic acid-treated group. Histopathological examination showed that the histopathological results of the tannic acid group were better than the group not given tannic acid. Biochemical and histopathological results showed that tannic acid administration had an antioxidant effect on the negative effects of ischemia in brain tissue.
许多关于脑缺血的研究表明，大量缺血诱导产生的活性氧在神经元死亡的主要机制中发挥了作用。然而，目前尚无预防活性氧引发细胞死亡的治疗方法。在我们的研究中，我们研究了抗氧化剂单宁酸对诱导性大脑中动脉闭塞大鼠缺血组织的影响。将动物分为三组，每组八只。假手术组仅腹腔注射10%乙醇，第二组诱导大脑中动脉闭塞并腹腔注射10%乙醇，而第三组诱导大脑中动脉闭塞并在半小时内腹腔注射溶解于10%乙醇中的10mg/kg剂量单宁酸。24小时后处死大鼠，对脑组织进行生化和组织病理学检查。脑组织的生化评估发现，将未治疗的缺血组与单宁酸治疗的缺血组进行比较；单宁酸治疗组的超氧化物歧化酶（SOD）水平较高，丙二醛（MDA）水平较低，核呼吸因子-1（NRF-1）较高。组织病理学检查表明，单宁酸组的组织病理学结果优于未给予单宁酸的组。生化和组织病理学结果表明，给予单宁酸对脑组织缺血的负面影响具有抗氧化作用。
