Interaction of gold nanoparticles with Doxorubicin mediated by supramolecular chemistry.

A copolymer containing β-cyclodextrin, catechol and polyethylene glycol groups in its side chain was designed for the in situ synthesis and coating of gold nanoparticles (Au@PEG-CD NPs). These platforms were designed as a smart carrier and traceable delivery probe of the chemotherapeutic Doxorubicin drug (Dox). The coated polymer forms stable complexes with Dox in water with a high binding constant (K=2.3×10(4) M(-1) at 25°C), which is one hundred times greater than those reported for its complexation with native βCD. Therefore, Au@PEG-CD NPs were able to load 0.01 mg of the drug per mg of NP and to release up to 60% of it in 48 h at 37°C. In addition, Au@PEG-CD NPs had the capacity to act as a quencher of Dox fluorescence when it was complexed with βCD in the NP organic shell. This feature allows the Dox release to be tracked by monitoring the recovery of its fluorescence in real time. Therefore, the Dox release kinetics and the influence of temperature on the thermal stability of Dox/CD complexes on Au@PEG-CD NP were investigated. The increase in temperature favors the dissociation of the complexes and subsequent Dox release from the NP. The first order rate constant for drug releasing was 1.1×10(-2) min(-1) with a half-life time of 63 min at 37°C. Finally, the great potential of the carrier/probe double nature of Au@PEG-CD NPs was demonstrated in real time inside HeLa cells.