Pang Ya-Yan, Chen Xin-Yi, Xue Yan, Han Xiao-Hua, Chen Lei
Department of Physiology, Faculty of Medicine, Qingdao University, Qingdao 266071, China.
Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China.
Peptides. 2015 Apr;66:1-8. doi: 10.1016/j.peptides.2015.01.012. Epub 2015 Feb 16.
Previous studies have shown that secretin and secretin receptors are expressed in central amygdala neurons. By using both in vivo extracellular recording as well as behavioral test, we investigated the direct electrophysiological effects of secretin in the central amygdala and its involvement in feeding behavior. Micro-pressure ejection of secretin increased the spontaneous firing rate by 104.22±26.18% in 13 out of the 27 central amygdala neurons. In other 6 out of the 27 neurons, secretin decreased the firing rate by 68.80±12.10%. Firing patter analysis showed that secretin did not change the firing pattern significantly. Further electrophysiological recordings revealed that secretin decreased the firing rate of glucose-sensitive neurons. In behavioral test, microinjection of secretin into the central amygdala significantly reduced cumulative food intake through cAMP-activated protein kinase activation. Based on the present electrophysiological and behavioral findings, we hypothesized that secretin may suppress food intake by its modulation of spontaneous firing of central amygdala neurons.
先前的研究表明，促胰液素和促胰液素受体在中央杏仁核神经元中表达。通过使用体内细胞外记录以及行为测试，我们研究了促胰液素在中央杏仁核中的直接电生理效应及其在进食行为中的作用。在27个中央杏仁核神经元中的13个中，微量压力注射促胰液素使自发放电率增加了104.22±26.18%。在27个神经元中的另外6个中，促胰液素使放电率降低了68.80±12.10%。放电模式分析表明，促胰液素没有显著改变放电模式。进一步的电生理记录显示，促胰液素降低了葡萄糖敏感神经元的放电率。在行为测试中，向中央杏仁核微量注射促胰液素通过激活环磷酸腺苷（cAMP）激活的蛋白激酶显著减少了累积食物摄入量。基于目前的电生理和行为学发现，我们推测促胰液素可能通过调节中央杏仁核神经元的自发放电来抑制食物摄入。
