Cong Luyen Huynh, Dauwe Rebecca, Lequart Michelle, Vinchon Sophie, Renouard Sullivan, Fliniaux Ophélie, Colas Cyril, Corbin Cyrielle, Doussot Joël, Hano Christophe, Lamblin Frédéric, Molinié Roland, Pilard Serge, Jullian Nathalie, Boitel Michèle, Gontier Eric, Mesnard François, Laberche Jean-Claude
Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France; Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
Phytochemistry. 2015 Jul;115:70-8. doi: 10.1016/j.phytochem.2015.01.001. Epub 2015 Feb 16.
Due to their pronounced cytotoxic activity, a number of aryltetralin lignans (ATLs), such as podophyllotoxin (PTOX), are used as antitumor compounds. The production of such molecules from entire plants or plant cell-tissue-organ cultures is thus of interest to the pharmaceutical industry. Hairy root cultures constitute a good tool not only for phytochemical production but also for investigating plant secondary metabolism. This work reports on the growth and ATL biosynthesis in two hairy root cultures of Linum album Kotschy ex Boiss. and Linum flavum. The kinetics of accumulation of the intermediates of MPTOX biosynthesis and of their glucosylated forms are described over a 21-day period of growth. An accumulation of non-glucosylated forms of the ATLs during the exponential phase of the cultures is followed by an accumulation of the glucosylated forms during the stationary phase. Our results show a strong coordination of the biosynthetic paths derived from deoxypodophyllotoxin via deoxypodophyllotoxin 6-hydroxylase and deoxypodophyllotoxin 7-hydroxylase, and a coordinated glucosylation of podophyllotoxin, methoxypodophyllotoxin, and 5'-demethoxymethoxypodophyllotoxin. Furthermore, our results suggest an important role of β-peltatin-6-glucoside formation in the control of ATL accumulation in Linum hairy root cultures.
由于其显著的细胞毒性活性，一些芳基四氢萘木脂素（ATLs），如鬼臼毒素（PTOX），被用作抗肿瘤化合物。因此，从整株植物或植物细胞-组织-器官培养物中生产此类分子受到制药行业的关注。毛状根培养不仅是植物化学物质生产的良好工具，也是研究植物次生代谢的良好工具。这项工作报道了白亚麻（Linum album Kotschy ex Boiss.）和金黄亚麻（Linum flavum）两种毛状根培养物的生长和ATL生物合成。描述了MPTOX生物合成中间体及其糖基化形式在21天生长期间的积累动力学。在培养的指数期，ATLs的非糖基化形式积累，随后在稳定期糖基化形式积累。我们的结果表明，通过脱氧鬼臼毒素6-羟化酶和脱氧鬼臼毒素7-羟化酶，源自脱氧鬼臼毒素的生物合成途径具有很强的协调性，并且鬼臼毒素、甲氧基鬼臼毒素和5'-去甲氧基甲氧基鬼臼毒素存在协同糖基化作用。此外，我们的结果表明，β-盾叶鬼臼素-6-葡萄糖苷的形成在亚麻毛状根培养物中ATL积累的控制中起重要作用。
