Laboratoire de Biologie des Ligneux et des Grandes Cultures, LBLGC EA1207/INRA USC1328, Team Plant Lignans, Université d'Orléans, 28000 Chartres, France.
Ecole Sciences Industrielles et Technologies de l'Information, SITI Département CASER, Le CNAM, 75141 Paris, France.
Int J Mol Sci. 2018 Mar 26;19(4):990. doi: 10.3390/ijms19040990.
hairy root lines were established from hypocotyl pieces using strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of as potential sources for these valuable metabolites as an alternative, or as a complement to collected from wild stands.
发根培养系由子叶片段利用 LBA9402 和 ATCC15834 菌株建立。两种菌株都能有效地进行转化，但 ATCC15834 菌株诱导的毛状根表型更稳定。虽然在鬼臼毒素相关化合物（6-甲氧基鬼臼毒素、鬼臼毒素和脱氧鬼臼毒素）中观察到类似的积累模式，但在根系之间存在显著的定量差异。研究了培养基和各种处理（激素、诱导和前体喂养）的影响。在 Gamborg B5 培养基中获得了最高的积累。用茉莉酸甲酯处理和用阿魏酸喂养增加了芳基四氢萘木脂素的积累。这些结果表明，可以将 的发根培养系作为这些有价值代谢物的潜在来源，作为替代物，或作为从野生种群中收集的 的补充。
