Omoto E, Tavassoli M
University of Mississippi School of Medicine, Jackson.
Biochem Biophys Res Commun. 1989 Aug 15;162(3):1346-50. doi: 10.1016/0006-291x(89)90821-8.
Through a process resembling receptor-mediated endocytosis, liver endothelium binds and internalizes the plasma glycoprotein ceruloplasmin (CP) on the luminal side. The protein is then transported via a vesicular system to the albuminal side where it is externalized to the space of Disse. In its path, the glycoprotein is fully desialylated. To determine if the endosomal compartment is involved in this transport, we used endosomal inhibitors NH4Cl, ethylamine as well as monensin to quantitatively measure the magnitude of radiolabeled CP transport across purified liver endothelial cells. All three reagents inhibited the transport of CP and its discharge by endothelium. The magnitude of inhibition was dose-related for all three reagents. We conclude that the endosomal compartment is involved in the transendothelial transport of CP across the liver endothelium.
通过一个类似于受体介导的内吞作用的过程，肝内皮细胞在管腔侧结合并内化血浆糖蛋白铜蓝蛋白（CP）。然后该蛋白通过囊泡系统运输到白蛋白侧，在那里它被释放到狄氏间隙。在其运输过程中，糖蛋白完全去唾液酸化。为了确定内体区室是否参与这种运输，我们使用内体抑制剂氯化铵、乙胺以及莫能菌素定量测量放射性标记的CP跨纯化肝内皮细胞运输的幅度。所有这三种试剂都抑制了CP的运输及其被内皮细胞释放。对于所有这三种试剂，抑制幅度都与剂量相关。我们得出结论，内体区室参与了CP跨肝内皮细胞的跨内皮运输。
