Irie S, Tavassoli M
Biochem Biophys Res Commun. 1986 Oct 15;140(1):94-100. doi: 10.1016/0006-291x(86)91062-4.
Our previous work indicated that ceruloplasmin (CP) is transported through liver endothelium via a receptor-mediated mechanism and is subsequently externalized on the abluminal side. In the present work 125I or 3H-labeled ceruloplasmin was chased through the endothelium and the supernate was subjected to affinity chromatography using RCA120 column. Evidence was obtained that liver endothelium completely desialates CP in the course of its transport. The results substantiated the view that desialated CP is subsequently removed by hepatocytes through asialoglycoprotein receptors.
我们之前的研究表明，铜蓝蛋白（CP）通过受体介导的机制穿过肝内皮细胞，并随后在管腔外侧外化。在本研究中，将125I或3H标记的铜蓝蛋白追踪穿过内皮细胞，然后将上清液用RCA120柱进行亲和层析。有证据表明，肝内皮细胞在其运输过程中会完全去除CP上的唾液酸。这些结果证实了这样一种观点，即去唾液酸化的CP随后会被肝细胞通过去唾液酸糖蛋白受体清除。
