四种未充分利用的豆科植物的转录组测序与标记开发
Transcriptome sequencing and marker development for four underutilized legumes.
作者信息
Chapman Mark A
机构信息
Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom ; Centre for Underutilised Crops, University of Southampton, Southampton SO17 1BJ, United Kingdom.
出版信息
Appl Plant Sci. 2015 Feb 10;3(2). doi: 10.3732/apps.1400111. eCollection 2015 Feb.
UNLABELLED
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PREMISE OF THE STUDY
Combating threats to food and nutrition security in the context of climate change and global population increase is one of the highest priorities of major international organizations. Hundreds of species are grown on a small scale in some of the most drought/flood-prone regions of the world and as such may harbor some of the most environmentally tolerant crops (and alleles). •
METHODS AND RESULTS
In this study, transcriptomes were sequenced, assembled, and annotated for four underutilized legume crops. Microsatellite markers were identified in each species, as well as a conserved orthologous set of markers for cross-family phylogenetics and comparative mapping, which were ground-truthed on a panel of diverse legume germplasm. •
CONCLUSIONS
An understanding of these underutilized legumes will inform crop selection and breeding by allowing the investigation of genetic variation and the genetic basis of adaptive traits to be established.
未标注
研究前提：在气候变化和全球人口增长的背景下，应对粮食和营养安全面临的威胁是主要国际组织的首要任务之一。数百种物种在世界上一些最易遭受干旱/洪水的地区小规模种植，因此可能蕴藏着一些对环境耐受性最强的作物（及等位基因）。
方法与结果：在本研究中，对四种未充分利用的豆科作物进行了转录组测序、组装和注释。在每个物种中鉴定了微卫星标记，以及用于跨家族系统发育和比较图谱分析的一组保守直系同源标记，并在一组多样的豆科种质资源上进行了验证。
结论：对这些未充分利用的豆科植物的了解将有助于作物选择和育种，因为可以对遗传变异和适应性性状的遗传基础进行研究。
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