Clustal Omega，用于对大量序列进行精确比对。

Clustal Omega, accurate alignment of very large numbers of sequences.

作者信息

Sievers Fabian, Higgins Desmond G

机构信息

Conway Institute, University College Dublin, Dublin, Ireland.

出版信息

Methods Mol Biol. 2014;1079:105-16. doi: 10.1007/978-1-62703-646-7_6.

DOI:10.1007/978-1-62703-646-7_6

PMID:24170397

Abstract

Clustal Omega is a completely rewritten and revised version of the widely used Clustal series of programs for multiple sequence alignment. It can deal with very large numbers (many tens of thousands) of DNA/RNA or protein sequences due to its use of the mBED algorithm for calculating guide trees. This algorithm allows very large alignment problems to be tackled very quickly, even on personal computers. The accuracy of the program has been considerably improved over earlier Clustal programs, through the use of the HHalign method for aligning profile hidden Markov models. The program currently is used from the command line or can be run on line.

摘要

Clustal Omega是广泛使用的用于多序列比对的Clustal系列程序的完全重写和修订版本。由于它使用mBED算法来计算引导树，因此能够处理非常大量（数万条）的DNA/RNA或蛋白质序列。该算法允许即使在个人计算机上也能非常快速地解决非常大的比对问题。通过使用HHalign方法来比对轮廓隐马尔可夫模型，该程序的准确性比早期的Clustal程序有了显著提高。该程序目前可通过命令行使用，也可以在线运行。