Thrush G R, Butch A W, Lerman S P
Wayne State University School of Medicine, Department of Immunology and Microbiology, Detroit, Michigan 48201.
Cell Immunol. 1989 Sep;122(2):555-62. doi: 10.1016/0008-8749(89)90101-9.
CD8 cells, flow cytometrically sorted from the lymph nodes of tumor-bearing and normal SJL/J mice, suppressed in vitro proliferation of syngeneic CD4 cells in response to concanavalin A, two independent SJL/J lymphomas, and LPS-activated syngeneic B-cell blasts. The data confirm earlier reports that nonspecific suppressor cells are generated as a consequence of SJL/J lymphoma-stimulated T-cell proliferation. Earlier reports are extended, in that the suppressor cell is identified as expressing CD8, and the suppressor activity is shown to decrease the tumor-stimulated CD4 cell proliferation which is essential to growth of these CD4-dependent murine B-cell lymphomas. In three separate experiments, anti-CD8 treatment of mice, in which CD4 cells were made limiting by injection with anti-CD4, increased growth of transplantable SJL/J lymphomas with corresponding increases in numbers of CD4 cells. The data imply that, under certain conditions, CD8 suppressor cells measurably influence growth of SJL/J lymphomas by regulating the tumor-stimulated CD4 cell proliferation essential to maximum growth of SJL/J lymphomas.
从荷瘤SJL/J小鼠和正常SJL/J小鼠的淋巴结中通过流式细胞术分选得到的CD8细胞，在体外抑制了同基因CD4细胞对伴刀豆球蛋白A、两种独立的SJL/J淋巴瘤以及脂多糖激活的同基因B细胞母细胞的增殖反应。这些数据证实了早期的报道，即非特异性抑制细胞是SJL/J淋巴瘤刺激T细胞增殖的结果。早期报道得到了扩展，因为抑制细胞被鉴定为表达CD8，并且抑制活性被证明可降低肿瘤刺激的CD4细胞增殖，而这种增殖对于这些依赖CD4的小鼠B细胞淋巴瘤的生长至关重要。在三个独立的实验中，对通过注射抗CD4使CD4细胞数量受限的小鼠进行抗CD8治疗，可增加可移植SJL/J淋巴瘤的生长，同时CD4细胞数量相应增加。这些数据表明，在某些条件下，CD8抑制细胞通过调节对SJL/J淋巴瘤最大生长至关重要的肿瘤刺激的CD4细胞增殖，可显著影响SJL/J淋巴瘤的生长。
