粗糙脉孢菌线粒体伴侣蛋白寡聚体的鉴定及电子显微镜分析

Hutchinson E G, Tichelaar W, Hofhaus G, Weiss H, Leonard K R

European Molecular Biology Laboratory, Heidelberg, FRG.

EMBO J. 1989 May;8(5):1485-90. doi: 10.1002/j.1460-2075.1989.tb03532.x.

A 7-fold symmetric particle has been identified in Neurospora crassa which is most probably the mitochondrial chaperonin. The particle, about 12 nm in diameter, appears in preparations of cytochrome reductase, and is shown to contain a 60 kd protein which cross-reacts with anti-GroEL antibodies. Results of STEM mass measurement suggest that the particle is composed of 14 subunits. A preliminary interpretation of the structure of the particle based on electron microscopy is given. Its quaternary structure and molecular weight are similar to those of the recently discovered family of particles called chaperonins, found in bacteria, chloroplasts and mitochondria.

在粗糙脉孢菌中发现了一种七重对称颗粒，它很可能是线粒体伴侣蛋白。该颗粒直径约12纳米，出现在细胞色素还原酶制剂中，并且显示含有一种与抗GroEL抗体发生交叉反应的60kd蛋白。扫描透射电子显微镜质量测量结果表明该颗粒由14个亚基组成。基于电子显微镜给出了该颗粒结构的初步解释。它的四级结构和分子量与最近在细菌、叶绿体和线粒体中发现的称为伴侣蛋白的颗粒家族相似。