Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
J Agric Food Chem. 2015 Apr 8;63(13):3437-44. doi: 10.1021/jf506120w. Epub 2015 Mar 25.
The objective of this study was to prepare antioxidant peptides from duck meat hydrolysate (DMH) using Protamex. The DPPH(•) scavenging activity, hydroxyl radical ((•)OH) scavenging activity, and Fe(2+)-chelating ability of DMH were investigated. DMH was separated into three groups, MWCO-1 (69.57%), MWCO-2 (9.53%), and MWCO-3 (8.21%), by ultrafiltration. MWCO-3 exhibited the highest DPPH(•) scavenging activity (83.17 ± 0.73%) and was subsequently fractionated by using gel filtration chromatography to obtain fraction B (40.90%). Fraction B5 (6.71%) obtained from ion exchange chromatography exhibited the highest DPPH(•) scavenging activity (93.63 ± 0.13%) and contained seven peptides which were characterized by LC-MS/MS. Among these peptides, LQAEVEELRAALE showed the highest DPPH(•) scavenging activity (93.36 ± 0.53%) and Fe(2+)-chelating ability (87.13 ± 0.47%) and IEDPFDQDDWGAWKK exhibited the highest (•)OH scavenging activity (46.51 ± 0.16%). The results presented here indicated that DMH could serve as a suitable source of antioxidant peptides.
本研究旨在利用 Protamex 从鸭肉水解物（DMH）中制备抗氧化肽。研究了 DMH 的 DPPH（•）清除活性、羟基自由基（（•）OH）清除活性和 Fe（2+）螯合能力。DMH 通过超滤分为三组，MWCO-1（69.57%）、MWCO-2（9.53%）和 MWCO-3（8.21%）。MWCO-3 表现出最高的 DPPH（•）清除活性（83.17±0.73%），随后通过凝胶过滤色谱进一步分离，得到 B 馏分（40.90%）。从离子交换色谱获得的 B5 馏分（6.71%）表现出最高的 DPPH（•）清除活性（93.63±0.13%），并含有七种通过 LC-MS/MS 鉴定的肽。在这些肽中，LQAEVEELRAALE 表现出最高的 DPPH（•）清除活性（93.36±0.53%）和 Fe（2+）螯合能力（87.13±0.47%），IEDPFDQDDWGAWKK 表现出最高的（•）OH 清除活性（46.51±0.16%）。本研究结果表明，DMH 可以作为抗氧化肽的合适来源。
