Key Laboratory of Meat Processing and Quality Control, Key Laboratory of Meat Processing, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China; Nanjing Professor Huang Food Science and Technology Co. Ltd., Nanjing, Jiangsu 211225, People's Republic of China.
College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Food Chem. 2020 Jul 30;319:126534. doi: 10.1016/j.foodchem.2020.126534. Epub 2020 Mar 2.
The antioxidant peptides extracted from duck plasma hydrolysate (DPH) was investigated. The antioxidant activity of DPH, which was isolated and purified via ultrafiltration, size exclusion chromatography, and reversed-phase high-performance liquid chromatography, was evaluated using its free radical scavenging ability. Nano-liquid chromatography-tandem mass spectrometry was conducted to identify the DPH fractions with the highest antioxidant ability. Seven novel peptides: LDGP, TGVGTK, EVGK, RCLQ, LHDVK, KLGA, and AGGVPAG (400.43, 561.63, 431.48, 260.14, 610.71, 387.47, and 527.57 Da, respectively) were identified and synthesized using a solid-phase peptide produce to evaluate their antioxidant activities. Of these, EVGK exhibited the highest Fe chelating ability (16.35%), and RCLQ presented the highest reducing power, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt scavenging activity, and 1,1-diphenyl-2-picrylhydrazyl scavenging rate (0.62, 274.83 mM TE/mg, and 95.12%, respectively). Our results indicated that DPH possessed antioxidant capabilities and could be used to obtain antioxidant peptides, thus adding economic value to duck blood.
从鸭血浆水解物（DPH）中提取抗氧化肽。通过超滤、分子筛层析和反相高效液相色谱对 DPH 进行分离和纯化，评估其自由基清除能力来研究其抗氧化活性。采用纳流液相色谱-串联质谱法鉴定具有最强抗氧化能力的 DPH 级分。使用固相肽合成法鉴定并合成了 7 种新型肽：LDGP、TGVGTK、EVGK、RCLQ、LHDVK、KLGA 和 AGGVPAG（分别为 400.43、561.63、431.48、260.14、610.71、387.47 和 527.57 Da），并评估其抗氧化活性。其中，EVGK 的铁螯合能力最高（16.35%），而 RCLQ 的还原能力、2,2-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐清除活性和 1,1-二苯基-2-苦基肼清除率最高（0.62、274.83 mM TE/mg 和 95.12%）。结果表明，DPH 具有抗氧化能力，可用于获得抗氧化肽，从而为鸭血增加经济价值。
