Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Biomaterials. 2015 Apr;48:119-28. doi: 10.1016/j.biomaterials.2015.01.032. Epub 2015 Feb 11.
Intercellular interactions are important in the development, immune responses, and functions of tissues and organs. Few methods are currently available for experimentally inducing and analysing cell-cell interaction in vitro. Here I propose a method to induce cell-cell attachment by cell surface modification with single-stranded DNA (ssDNA) and poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) derivatives. The incorporation of an ssDNA pair (polyA20 and polyT20) into the cell membranes of two different cells was utilized to attach distinct cells through DNA hybridization. This technique enabled induction of cell-cell attachment between the same cell lines or different cell lines by controlling the contact area of two cells. Using this method, I investigated intercellular interactions, including the cell-in-cell invasion process, without impairing the interaction. I found that the normal cells MCF-10A were internalized into the cancer cells MCF-7, and that the intercellular interactions between them mainly involved the interaction of E-cadherin.
细胞间相互作用在组织和器官的发育、免疫反应和功能中非常重要。目前，很少有方法可以在体外实验性地诱导和分析细胞间相互作用。在这里，我提出了一种利用单链 DNA（ssDNA）和聚乙二醇化磷脂（PEG-脂质）衍生物对细胞表面进行修饰来诱导细胞-细胞附着的方法。将一对 ssDNA（polyA20 和 polyT20）整合到两种不同细胞的细胞膜中，通过 DNA 杂交将不同的细胞连接起来。通过控制两个细胞的接触面积，该技术可以诱导相同或不同细胞系之间的细胞-细胞附着。使用这种方法，我研究了细胞间的相互作用，包括细胞内入侵过程，而不会损害相互作用。我发现正常细胞 MCF-10A 被内化到癌细胞 MCF-7 中，它们之间的细胞间相互作用主要涉及 E-钙黏蛋白的相互作用。
