Teramura Yuji, Ekdahl Kristina N, Barbu Andreea
Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Immunology, Genetics and Pathology (IGP), Uppsala University, Dag Hammarskjölds väg 20, SE-751 85 Uppsala, Sweden.
Regen Ther. 2016 Mar 19;3:68-74. doi: 10.1016/j.reth.2016.03.004. eCollection 2016 Mar.
Cell surface engineering using single-stranded DNA-poly(ethylene glycol)-conjugated phospholipid (ssDNA-PEG-lipid) is useful for inducing cell-cell attachment two and three dimensionally. In this review, we summarize our recent techniques for cell surface engineering and their applications to islet transplantation. Because any DNA sequence can be immobilized onto the cell surface by hydrophobic interactions between ssDNA-PEG-lipid and the cellular membrane without impairing cell function, a cell-cell hybrid can be formed through the DNA hybridization. With this technique, it would be possible to create three-dimensional hybrid structures of pancreatic islets coated with various accessory cells, such as patients' own cells, mesenchymal and adipose-derived stem cells, endothelial progenitor cells, neural crest stem cells or regulatory T cells, which might significantly improve the outcome of islet transplantation in diabetic patients.
使用单链DNA-聚乙二醇-共轭磷脂（ssDNA-PEG-脂质）进行细胞表面工程，对于在二维和三维空间诱导细胞间附着很有用。在这篇综述中，我们总结了我们最近的细胞表面工程技术及其在胰岛移植中的应用。由于任何DNA序列都可以通过ssDNA-PEG-脂质与细胞膜之间的疏水相互作用固定在细胞表面，而不会损害细胞功能，因此可以通过DNA杂交形成细胞-细胞杂交体。通过这项技术，有可能创建由各种辅助细胞包被的胰岛三维杂交结构，这些辅助细胞如患者自身细胞、间充质和脂肪来源的干细胞、内皮祖细胞、神经嵴干细胞或调节性T细胞，这可能会显著改善糖尿病患者胰岛移植的效果。
