Castilla J A, Rueda R, Vargas M L, González-Gómez F, García-Olivares E
Department of Biochemistry and Molecular Biology, University Hospital, Faculty of Medicine, Granada, Spain.
J Reprod Immunol. 1989 May;15(2):103-11. doi: 10.1016/0165-0378(89)90030-2.
Peripheral blood mononuclear cell (PBMC) populations during human pregnancy have been investigated by many authors, although the different results obtained, principally in relation to T cells, are very discrepant. In this study we aimed to exclude all the possible causes of these discrepancies: small sample size; diurnal rhythm of CD4+ T cells; smoking habits; haemodilution which occurs during pregnancy and inappropriate statistical analysis; in order to determine whether gestation has a definite effect on PBMC populations. We found that the percentage of CD4+ T lymphocytes decreases in the first and second trimesters, returns to the non-pregnant level in the third trimester and remains there in the postpartum period. The percentages of CD3+ T lymphocytes run parallel to those of CD4+ while CD8+ T lymphocytes do not vary. The proportion of CD16+ cells, which include mature NK cells, diminishes in the second trimester and this reduction is maintained in the third trimester and the puerperium. No variation was found in the other PBMC studied (CD20+ lymphocytes, CD14+ monocytes and D/DR+ cells). When parity was considered no difference was seen between primiparous and multiparous women in any of the cell populations tested.
许多作者对人类孕期外周血单个核细胞（PBMC）群体进行了研究，尽管主要关于T细胞所获得的不同结果差异很大。在本研究中，我们旨在排除这些差异的所有可能原因：样本量小；CD4⁺ T细胞的昼夜节律；吸烟习惯；孕期发生的血液稀释以及不恰当的统计分析；以便确定妊娠是否对PBMC群体有明确影响。我们发现，CD4⁺ T淋巴细胞百分比在妊娠头三个月和第二个三个月下降，在第三个三个月恢复到非妊娠水平，并在产后保持在该水平。CD3⁺ T淋巴细胞百分比与CD4⁺ T淋巴细胞百分比变化趋势平行，而CD8⁺ T淋巴细胞无变化。包括成熟NK细胞在内的CD16⁺细胞比例在第二个三个月减少，且在第三个三个月和产褥期维持这种减少。在所研究的其他PBMC（CD20⁺淋巴细胞、CD14⁺单核细胞和D/DR⁺细胞）中未发现变化。当考虑产次时，在任何测试的细胞群体中，初产妇和经产妇之间均未观察到差异。
