日本人群X染色体q24 - q28区域的限制性片段长度多态性

Restriction fragment length polymorphisms on the q24-q28 region of X chromosome among Japanese population.

作者信息

Taga T, Shirahase W, Shimada M, Kurokawa K, Ueyama H

出版信息

Jinrui Idengaku Zasshi. 1989 Jun;34(2):123-8. doi: 10.1007/BF01912480.

DOI:10.1007/BF01912480

Abstract

Restriction fragment length polymorphisms were studied among the Japanese population using 12 polymorphic DNA probes on the q24-q28 region of X chromosome. The frequency distribution for probes p22-33, p482.6a, p43-15, 52A, pPM101, cX33.2 and cpx234, was the same as that for Caucasians, and that for probes 4D-8 and St14-1 (MspI) was slightly different (p less than 0.05). However, it was quite different (p less than 0.01) for probes p114.12, St14-1 (TaqI), 36B-2 and MN12. Probe p114.12 showed no HindIII polymorphism for the Japanese people. On the contrary, probe MN12, which has a low PIC value (0.15) for Caucasians, was found to be useful for Japanese (PIC value = 0.50). These results suggest that 7 DNA probes (p482.6a, p43-15, 52A, St14-1, p114.12 (BclI), 36B-2 and MN12) are useful (PIC greater than 0.42) for linkage analysis of X-linked disease in Japan.

摘要

利用12种多态性DNA探针，对日本人群X染色体q24 - q28区域的限制性片段长度多态性进行了研究。探针p22 - 33、p482.6a、p43 - 15、52A、pPM101、cX33.2和cpx234的频率分布与高加索人相同，而探针4D - 8和St14 - 1（MspI）的频率分布略有不同（p小于0.05）。然而，探针p114.12、St14 - 1（TaqI）、36B - 2和MN12的频率分布差异很大（p小于0.01）。探针p114.12在日本人中未显示HindIII多态性。相反，探针MN12对高加索人而言多态信息含量（PIC）值较低（0.15），但对日本人却很有用（PIC值 = 0.50）。这些结果表明，7种DNA探针（p482.6a、p43 - 15、52A、St14 - 1、p114.12（BclI）、36B - 2和MN12）在日本对于X连锁疾病的连锁分析很有用（PIC大于0.42）。