Li Jun, Ban Liping, Wen Hongyu, Wang Zan, Dzyubenko Nikolay, Chapurin Vladimir, Gao Hongwen, Wang Xuemin
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China; Institute of Grassland Research, Chinese Academy of Agricultural Sciences / SharaQin Key Wild Scientific Monitoring Station for Forage Resources of Ministry of Agriculture, 120 Wulanchabu East Street, Saihan District, Hohhot 010010, People's Republic of China.
College Animal Science & Technology, China Agricultural University, Beijing 100193, People's Republic of China.
Biochem Biophys Res Commun. 2015 Apr 3;459(2):208-213. doi: 10.1016/j.bbrc.2015.02.052. Epub 2015 Feb 18.
Water channel proteins known as aquaporins (AQPs) regulate the movement of water and other small molecules across plant vacuolar and plasma membranes; they are associated with plant tolerance of biotic and abiotic stresses. In this study, a PIP type AQPs gene, designated as GoPIP1, was cloned from Galega orientalis, a high value leguminous forage crop. The GoPIP1 gene consists of an 870 bp open reading frame encoding a protein of 289 amino acids, and belongs to the PIP1 subgroup of the PIP subfamily. The transcript level of GoPIP1 was higher in the root of G. orientalis than in the leaf and stem. The level of GoPIP1 transcript increased significantly when treated with 200 mM NaCl or 20% polyethylene glycol (PEG) 6000. Transient expression of GoPIP1 in onion epidermal cells revealed that the GoPIP1 protein was localized to the plasma membrane. Over-expression of GoPIP1 increased the rosette/root ratio and increased sensitivity to drought in transgenic Arabidopsis plants. However, GoPIP1 over-expression in Arabidopsis had no significant effect under saline condition. The present data provides a gene resource that contributes to furthering our understanding of water channel protein and their application in plant stress tolerance.
被称为水通道蛋白（AQPs）的水通道蛋白调节水和其他小分子跨植物液泡膜和质膜的运动；它们与植物对生物和非生物胁迫的耐受性有关。在本研究中，从高价值豆科牧草东方山羊豆中克隆了一个PIP型水通道蛋白基因，命名为GoPIP1。GoPIP1基因由一个870 bp的开放阅读框组成，编码一个289个氨基酸的蛋白质，属于PIP亚家族的PIP1亚组。GoPIP1在东方山羊豆根中的转录水平高于叶和茎。用200 mM NaCl或20%聚乙二醇（PEG）6000处理后，GoPIP1转录水平显著增加。GoPIP1在洋葱表皮细胞中的瞬时表达表明，GoPIP1蛋白定位于质膜。GoPIP1的过表达增加了转基因拟南芥植株的莲座叶/根比，并增加了对干旱的敏感性。然而，在盐胁迫条件下，拟南芥中GoPIP1的过表达没有显著影响。目前的数据提供了一种基因资源，有助于进一步了解水通道蛋白及其在植物胁迫耐受性中的应用。
