Li Xin, Li Ci, Zhu Lu-hong
Zhonghua Nan Ke Xue. 2015 Jan;21(1):31-4.
To investigate the correlation of the autophagy-associated gene Atg5 with the pathogenesis of prostate cancer.
Using real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and immunohistochemistry, we detected the expression of Atg5 in 50 cases of prostate intraepithelial neoplasm (PIN), 69 cases of prostate cancer (PCa), and 30 cases of benign prostatic hyperplasia (BPH).
The expression level of Atg5 mRNA was significantly higher in PIN (5.270 ± 0.230) and PCa (5.131 ± 0.252) than in the BPH tissue (1.723 ± 0.017) (P <0.01), and so was the positive rate of the Atg5 expression in the patients of the PIN group (94%) and PCa group (88.4%) than in those of the BPH group (6.7%) (P<0.01), but with no statistically significant differences between the PIN and PCa groups (P >0.05). No significant correlation was observed between the expression of Atg5 and the Gleason score of PCa (P >0.05).
The upregulated expression of Atg5 might play a role in the tumorigenesis of prostate cancer.
探讨自噬相关基因Atg5与前列腺癌发病机制的相关性。
采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）和免疫组织化学方法，检测50例前列腺上皮内瘤变（PIN）、69例前列腺癌（PCa）及30例良性前列腺增生（BPH）组织中Atg5的表达情况。
PIN组织（5.270±0.230）和PCa组织（5.131±0.252）中Atg5 mRNA表达水平显著高于BPH组织（1.723±0.017）（P<0.01），PIN组（94%）和PCa组（88.4%）患者Atg5表达阳性率也显著高于BPH组（6.7%）（P<0.01），但PIN组与PCa组之间差异无统计学意义（P>0.05）。Atg5表达与PCa的Gleason评分无显著相关性（P>0.05）。
Atg5表达上调可能在前列腺癌的发生中起作用。
