胎球蛋白A、脂联素、白细胞介素10及总抗氧化能力与体外受精结局的相关性
Association of fetuin A, adiponectin, interleukin 10 and total antioxidant capacity with IVF outcomes.
作者信息
Yen Mustafa, Donma Orkide, Yildizfer Ferdag, Ekmekci Ozlem, Asli Karatas Kul Z, Esat Imal A, Keser Zafer, Cagil Emin, Mengi Murat, Ekmekci Hakan, Sahmay Sezai, Donma Metin
机构信息
Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
Ministry of Health, Diyarbakir Education and Research Hospital, Diyarbakir, Turkey.
出版信息
Iran J Reprod Med. 2014 Nov;12(11):747-54.
BACKGROUND
Possible roles of anti-inflammatory factors as well as total antioxidative capacity in reproductive processes of women undergoing in vitro fertilization (IVF) are still being investigated and the contributions by some of them remain controversial.
OBJECTIVE
The aim of this study is to investigate the relationship between anti-inflammatory parameters and total antioxidative capacity (TAC) of the body during IVF. In this respect, adiponectin, interleukin-10 (IL-10), interleukin-1 receptor antagonist (IL-1RA), fetuin A and TAC analyses have been performed.
MATERIALS AND METHODS
In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile (Group-1), and 26 infertile women before (Group-2) and after (Group-3) IVF treatment were analyzed. IL-1RA, IL-10, fetuin A, adiponectin and insulin were determined by ELISA. TAC was determined spectrophotometrically. Mann-Whitney U test, paired sample t-test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis.
RESULTS
Clinical pregnancy and live birth rates were determined as 30.8% and 23.1%, respectively, in pregnant group. For the pregnant, significant indirect correlations were detected between fetuin A and adiponectin (r=-0.843; p=0.035) as well as IL-10 (r=-0.846; p=0.034) in Group 2. The correlation between adiponectin and IL-10 doubled in pregnant compared to non-pregnant (r=0.929; p=0.007 vs. r=0.478; p=0.033). The correlations between fetuin A and TAC in pregnant were noted both in Group 2 (r=0.892; p=0.017) and Group 3 (r=0.875; p=0.022). No correlation of fetuin A with these parameters was detected in non-pregnant group.
CONCLUSION
Fetuin A, TAC, IL-10, adiponectin and their associations may be important from their predictive values for IVF success point of view. Parameters with anti-inflammatory or antioxidant property appear to improve pregnancy in women undergoing IVF.
背景
抗炎因子以及总抗氧化能力在接受体外受精（IVF）的女性生殖过程中的潜在作用仍在研究中，其中一些因子的作用仍存在争议。
目的
本研究旨在探讨IVF期间抗炎参数与机体总抗氧化能力（TAC）之间的关系。在此方面，已进行了脂联素、白细胞介素-10（IL-10）、白细胞介素-1受体拮抗剂（IL-1RA）、胎球蛋白A和TAC分析。
材料与方法
在这项前瞻性、非随机、对照临床研究中，分析了从26名可育女性（第1组）以及26名不孕女性在IVF治疗前（第2组）和治疗后（第3组）获得的血清。通过酶联免疫吸附测定法（ELISA）测定IL-1RA、IL-10、胎球蛋白A、脂联素和胰岛素。通过分光光度法测定TAC。使用SPSS进行曼-惠特尼U检验、配对样本t检验、威尔科克森符号秩检验以及皮尔逊相关分析进行统计分析。
结果
妊娠组的临床妊娠率和活产率分别确定为30.8%和23.1%。对于妊娠女性，在第2组中检测到胎球蛋白A与脂联素（r = -0.843；p = 0.035）以及IL-10（r = -0.846；p = 0.034）之间存在显著的间接相关性。与未妊娠女性相比，妊娠女性中脂联素与IL-10之间的相关性增加了一倍（r = 0.929；p = 0.007对r = 0.478；p = 0.033）。在第2组（r = 0.892；p = 0.017）和第3组（r = 0.875；p = 0.022）中均注意到妊娠女性中胎球蛋白A与TAC之间的相关性。在未妊娠组中未检测到胎球蛋白A与这些参数之间的相关性。
结论
从IVF成功的预测价值角度来看，胎球蛋白A、TAC、IL-10、脂联素及其关联可能很重要。具有抗炎或抗氧化特性的参数似乎可改善接受IVF的女性的妊娠情况。
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