Bódis József, Peti Attila Mihály, Sulyok Endre, Kovács Gábor L, Várnagy Akos
Clin Chem Lab Med. 2014 Sep;52(9):1313-8. doi: 10.1515/cclm-2014-0211.
This case-control study was designed to assess the possible role of fetuin-A, a multifunctional protein, in reproductive processes of women undergoing in vitro fertilization (IVF).
Paired serum and follicular fluid (FF) samples were obtained from 35 unselected patients who received IVF treatment. Their main clinical chartacteristics: age 35.2±5.4 years, BMI 22.9±2.8, duration of infertility 3.2±2.0 years, gonadotropin administered 1199.4±202.3 IU, and estradiol level on day 6 of stimulation 1827±1342 pmol/L. In total 25 healthy women of similar age admitted for minor elective surgery served as controls for serum analysis. Fetuin-A and high-sensitivity C-reactive protein (hsCRP) were measured in serum and FF by using commercially available ELISA kits and turbidimetric immunoassay, respectively.
Serum fetuin-A levels of patients participating IVF are markedly elevated when compared to those of healthy women (1.30±0.58 g/L vs. 0.55±0.09 g/L, p<0.000). Fetuin-A in FF proved to be comparable to its serum levels (1.17±0.45 g/L) and no relationship could be detected between the respective individual values. Moreover, markers of reproductive potential (number of oocytes and embryos) appeared to be independent of serum and FF fetuin-A. hsCRP in serum and FF was not related to fetuin-A and did not influence the number of oocytes and embryos.
Fetuin-A concentration is high in FF of patients undergoing IVF, however, it can not be used as an estimate of fertilization success.
本病例对照研究旨在评估多功能蛋白胎球蛋白-A在接受体外受精（IVF）的女性生殖过程中的可能作用。
从35例接受IVF治疗的未经过筛选的患者中获取配对的血清和卵泡液（FF）样本。她们的主要临床特征为：年龄35.2±5.4岁，体重指数22.9±2.8，不孕持续时间3.2±2.0年，促性腺激素用量1199.4±202.3 IU，刺激第6天的雌二醇水平1827±1342 pmol/L。总共25例因小型择期手术入院的年龄相仿的健康女性作为血清分析的对照。分别使用市售酶联免疫吸附测定（ELISA）试剂盒和比浊免疫测定法测定血清和FF中的胎球蛋白-A和高敏C反应蛋白（hsCRP）。
与健康女性相比，参与IVF的患者血清胎球蛋白-A水平显著升高（1.30±0.58 g/L对0.55±0.09 g/L，p<0.000）。FF中的胎球蛋白-A与其血清水平相当（1.17±0.45 g/L），且各自的个体值之间未发现相关性。此外，生殖潜能标志物（卵母细胞和胚胎数量）似乎与血清和FF中的胎球蛋白-A无关。血清和FF中的hsCRP与胎球蛋白-A无关，且不影响卵母细胞和胚胎数量。
接受IVF的患者FF中的胎球蛋白-A浓度较高，然而，它不能用于评估受精成功率。
