Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Front Med. 2017 Jun;11(2):247-252. doi: 10.1007/s11684-017-0519-6. Epub 2017 May 5.
The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IVembryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.
卵泡液中自由基的活性与卵巢反应性、体外受精（IVF）和胚胎移植成功率有关。然而，缺乏分析多囊卵巢综合征（PCOS）不孕妇女的自由基清除能力与胚胎质量之间关系的研究。本研究旨在评估 PCOS 妇女的卵泡液自由基清除窗与胚胎质量之间的关系。通过 a,a-二苯基-b-苦基肼（DPPH）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法、超氧自由基和活性氧（ROS）测定法来确定 PCOS 妇女卵泡液中的自由基清除能力。在 DPPH 和 ROS 测定中，I 级和 II 级胚胎的卵泡液明显高于 III 级和 IV 级胚胎的卵泡液。DPPH 自由基清除能力的下限和 ROS 水平的上限分别为 13.2%和 109.0 cps。在所有等级胚胎的卵泡液中进一步证实了计算出的 DPPH 自由基清除活性的下限和上限。这些卵泡液自由基清除活性的临界值可帮助胚胎学家选择接受 IVF 的 PCOS 患者胚胎的发育。
