富血小板血浆与脂肪来源干细胞联合使用对脂肪移植存活的影响。
The effect of combined use of platelet-rich plasma and adipose-derived stem cells on fat graft survival.
作者信息
Seyhan Nevra, Alhan Dogan, Ural Ali Ugur, Gunal Armagan, Avunduk Mustafa Cihad, Savaci Nedim
机构信息
From the *Department of Plastic and Reconstructive Surgery, Meram Medical School,Necmettin Erbakan University, Konya; †Department of Plastic and Reconstructive Surgery, ‡Department of Hematology, §Department of Pathology, Gulhane Military Academy, Ankara; and ∥Department of Pathology, Meram MedicalSchool, Necmettin Erbakan University, Konya, Turkey.
出版信息
Ann Plast Surg. 2015 May;74(5):615-20. doi: 10.1097/SAP.0000000000000480.
BACKGROUND
Free fat grafts have an unpredictable survival rate that limits their successful use. To increase the viability of fat grafts, it is important to minimize the reabsorption rate.
OBJECTIVE
Our aim was to investigate whether the combined use of platelet-rich plasma (PRP) and adipose derived stem cells (ADSCs) would contribute an improvement in lower resorption rates of fat grafts.
METHODS
Inbred Fischer 344 rats were randomized into 4 groups (n = 10). Fat grafts were mixed with Dulbecco modified Eagle medium in group A, with PRP in group B, with ADSC in group C, and with PRP + ADSC in group D and were injected to the scalp.In vitro growth factor (vascular endothelial growth factor, transforming growth factor-β, and fibroblast growth factor) levels were compared using enzyme-linked immunoassay method. After 12 weeks weight, volume and histology of the transplants were evaluated.
RESULTS
The mean weight and volume of the fat grafts were highest in group D. Histopathological investigations revealed that the number of viable adipocytes and blood vessels were highest in group D. The level of growth factors was significantly higher in stem cell plus PRP group.
CONCLUSION
Adipose-derived stem cells combined with PRP can enhance the survival of transplanted fat tissue.
背景
游离脂肪移植的成活率难以预测，这限制了其成功应用。为提高脂肪移植的存活率，尽量降低吸收率很重要。
目的
我们的目的是研究富血小板血浆（PRP）和脂肪来源干细胞（ADSCs）联合使用是否有助于降低脂肪移植的吸收率。
方法
将近交系Fischer 344大鼠随机分为4组（每组n = 10）。A组脂肪移植与杜氏改良 Eagle培养基混合，B组与PRP混合，C组与ADSCs混合，D组与PRP + ADSCs混合，然后注射到头皮。采用酶联免疫吸附测定法比较体外生长因子（血管内皮生长因子、转化生长因子-β和成纤维细胞生长因子）水平。12周后评估移植组织的重量、体积和组织学情况。
结果
D组脂肪移植的平均重量和体积最高。组织病理学研究显示，D组成活脂肪细胞和血管数量最多。干细胞加PRP组生长因子水平显著更高。
结论
脂肪来源干细胞与PRP联合使用可提高移植脂肪组织的存活率。
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