Pitkänen A, Valjakka A, Pääkkönen A, Sirviö J, Jousmäki V, Partanen J, Riekkinen P J
Department of Neurology and Clinical Neurophysiology, University of Kuopio, Finland.
Neuropeptides. 1989 Jul;14(1):1-9. doi: 10.1016/0143-4179(89)90027-9.
Cysteamine (CYS) is known to be a quite specific depletor of somatostatin in the rat brain. In the present study we investigated the effect of CYS (100 mg/kg, 300 mg/kg, subcutaneously) on levels of somatostatin-like immunoreactivity (SLI) in the brain and cerebrospinal fluid, on catecholamines in the cortex, and on spectral cortical electroencephalogram (EEG) of rat. SLI was decreased in both the cortex and the striatum (p less than 0.05) of CYS-treated rats, but no change was seen in SLI of CSF. Cortical levels of dopamine, noradrenaline and homovanillic acid were decreased (p less than 0.05) following administration of either dose of CYS. In EEG, during mobility both the frontal and occipital peak (Fp) and mean (Fm) frequencies were slowed (p less than 0.05). Frontally, the amplitude of the frequency bands 1.5-3Hz and 3-5Hz was increased (p less than 0.05). During immobility the Fp and Fm were also slowed. In frequency bands of 3-5Hz, 5-10Hz and 10-20Hz the amplitude was decreased (p less than 0.05), indicating that, in addition to theta frequency, the low voltage fast activity is also affected by CYS. According to our results, both the cortical intrinsic neurons containing somatostatin and also the ascending catecholaminergic systems are affected after the single administration of CYS concomittantly with, but not necessarily related to, changes in different frequency bands in EEG.
已知半胱胺（CYS）是大鼠脑中一种相当特异的生长抑素耗竭剂。在本研究中，我们调查了皮下注射CYS（100mg/kg、300mg/kg）对大鼠脑和脑脊液中生长抑素样免疫反应性（SLI）水平、皮质中儿茶酚胺以及皮质脑电图（EEG）的影响。CYS处理的大鼠皮质和纹状体中的SLI均降低（p<0.05），但脑脊液中的SLI未见变化。给予任一剂量的CYS后，皮质中多巴胺、去甲肾上腺素和高香草酸的水平均降低（p<0.05）。在EEG中，活动期间额叶和枕叶的峰值（Fp）和平均（Fm）频率均减慢（p<0.05）。在额叶，1.5 - 3Hz和3 - 5Hz频段的振幅增加（p<0.05）。静止期间Fp和Fm也减慢。在3 - 5Hz、5 - 10Hz和10 - 20Hz频段，振幅降低（p<0.05），这表明除了θ频率外，低电压快活动也受到CYS的影响。根据我们的结果，单次给予CYS后，含有生长抑素的皮质固有神经元以及上行儿茶酚胺能系统均受到影响，这与EEG中不同频段的变化同时发生，但不一定相关。
