Pitkänen A, Hyttinen J M, Riekkinen P J
Department of Neurology, University of Kuopio, Finland.
Neuropeptides. 1989 Jul;14(1):11-6. doi: 10.1016/0143-4179(89)90028-0.
Previous studies have shown that a somatostatin-depleting drug, cysteamine (CYS), suppresses kindled seizures. However, no data is available concerning the levels of somatostatin-like immunoreactivity (SLI) in the kindled rat brain after CYS administration. In the present study, we used radioimmunoassay to measure SLI in the frontal cortex, amygdala + piriform cortex, hippocampus, striatum and hypothalamus: 1) in control rats, 2) in amygdala-kindled rats decapitated 14 days after the last stimulus, and 3) in amygdala-kindled rats decapitated 14 days after the last stimulus but treated either 11 days or 4) 4 hours before decapitation with CYS (100 mg/kg, subcutaneously). The results showed that, compared to controls, in kindled rats SLI was elevated both in the ipsi lateral (28%, p = 0.0372) and contralateral (17%, p = 0.0078) frontal cortex. Compared to kindled rats, CYS given 4 hours before decapitation decreased SLI in the frontal cortex (to 71%, p = 0.0066) and hippocampus (to 72%, p = 0.0027), but compared to the controls, only in the hippocampus. In rats given CYS 11 days before decapitation, SLI did not differ from either the controls or from the kindled rats. In conclusion, the somatostatinergic system is affected in amygdala-kindling; but the relationship of anatomical localization and the magnitude of CYS-induced decrease of SLI to elevated seizure threshold needs to be studied further.
先前的研究表明，一种可消耗生长抑素的药物——半胱胺（CYS），能够抑制点燃性癫痫发作。然而，关于CYS给药后点燃大鼠脑内生长抑素样免疫反应性（SLI）水平的数据尚无报道。在本研究中，我们采用放射免疫分析法测定额叶皮质、杏仁核+梨状皮质、海马、纹状体和下丘脑的SLI：1）对照组大鼠；2）在末次刺激后14天断头的杏仁核点燃大鼠；3）在末次刺激后14天断头，但在断头前11天或4）4小时皮下注射CYS（100mg/kg）的杏仁核点燃大鼠。结果显示，与对照组相比，点燃大鼠同侧（28%，p=0.0372）和对侧（17%，p=0.0078）额叶皮质的SLI均升高。与点燃大鼠相比，断头前4小时给予CYS可使额叶皮质（降至71%，p=0.0066）和海马（降至72%，p=0.0027）的SLI降低，但与对照组相比，仅海马出现此情况。在断头前11天给予CYS的大鼠中，SLI与对照组或点燃大鼠相比均无差异。总之，杏仁核点燃可影响生长抑素能系统；但CYS诱导的SLI降低的解剖定位与幅度和癫痫阈值升高之间的关系仍需进一步研究。
