Department of Infectious Diseases.
Animal Resource Center.
J Infect Dis. 2015 Aug 15;212(4):542-51. doi: 10.1093/infdis/jiv099. Epub 2015 Feb 23.
An effective vaccine is urgently needed against the H7N9 avian influenza virus. We evaluated the immunogenicity and protective efficacy of a split-virion H7N9 vaccine with or without the oil-in-water adjuvants in ferrets.
Ferrets were vaccinated with 2 doses of unadjuvanted, MF59 or AS03-adjuvanted A/Shanghai/2/2013 (H7N9) vaccine, and the induction of antibodies to hemagglutinin (HA) or neuraminidase proteins was evaluated. Ferrets were then challenged with wild-type H7N9 virus to assess the vaccine's protective efficacy. The vaccine composition and integrity was also evaluated in vitro.
Adjuvanted vaccines stimulated robust serum antibody titers against HA and neuraminidase compared with the unadjuvanted vaccines. Although there was a difference in adjuvanticity between AS03 and MF59 at a lower dose (3.75 µg of HA), both adjuvants induced comparable antibody responses after 2 doses of 15 µg. On challenge, ferrets that received adjuvanted vaccines showed lower viral burden than the control or unadjuvanted vaccine group. In vitro examinations revealed that the vaccine contained visible split-virus particles and retained the native conformation of HA recognizable by polyclonal and monoclonal antibodies.
The adjuvanted H7N9 vaccines demonstrated superior immunogenicity and protective efficacy against H7N9 infection in ferrets and hold potential as a vaccination regimen.
迫切需要针对 H7N9 禽流感病毒的有效疫苗。我们评估了含有或不含有油包水佐剂的 H7N9 裂解病毒疫苗在雪貂中的免疫原性和保护效力。
雪貂接种 2 剂无佐剂、MF59 或 AS03 佐剂的 A/上海/2/2013（H7N9）疫苗，评估对血凝素（HA）或神经氨酸酶蛋白的抗体诱导。然后用野生型 H7N9 病毒对雪貂进行攻毒，评估疫苗的保护效力。还在体外评估了疫苗的组成和完整性。
与无佐剂疫苗相比，佐剂疫苗刺激了针对 HA 和神经氨酸酶的更强烈的血清抗体滴度。尽管在较低剂量（3.75 µg HA）时 AS03 和 MF59 的佐剂效力存在差异，但在 2 剂 15 µg 后两者均诱导了可比的抗体反应。攻毒后，接受佐剂疫苗的雪貂的病毒载量低于对照组或无佐剂疫苗组。体外检查显示疫苗含有可见的裂病毒颗粒，并保留了可被多克隆和单克隆抗体识别的 HA 的天然构象。
含佐剂的 H7N9 疫苗在雪貂中显示出针对 H7N9 感染的优越免疫原性和保护效力，有作为疫苗接种方案的潜力。
