Hatamoto Masashi, Saito Yayoi, Dehama Kazuya, Nakahara Nozomi, Kuroda Kyohei, Takahashi Masanobu, Yamaguchi Takashi
Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan E-mail:
Department of Civil and Environmental Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
Water Sci Technol. 2015;71(3):454-61. doi: 10.2166/wst.2015.016.
The anaerobic-anoxic sequence batch reactor (A2SBR) was applied to achieve nitrogen and phosphorus removal in an energy-saving sewage treatment system involving an up-flow anaerobic sludge blanket combined with a down-flow hanging sponge reactor to treat municipal sewage. After sludge acclimation, the A2SBR showed satisfactory denitrification and phosphorus removal performance with total phosphate and nitrate concentrations of the effluent of 8.4 ± 3.4 mg-N L⁻¹ and 0.9 ± 0.6 mg-P L⁻¹, respectively. 16S rRNA gene sequence and fluorescence in situ hybridization analyses revealed that 'Candidatus Accumulibacter phosphatis' was the dominant phosphate-accumulating micro-organism. Although a competitive bacterium for polyphosphate-accumulating organisms, 'Ca. Competibacter phosphatis', was not detected, Dechloromonas spp. were abundant. The ppk1 gene sequence analysis showed that the type II lineage of 'Ca. Accumulibacter' was dominant. The results suggest that denitrification and phosphorus removal in the A2SBR could be achieved by cooperative activity of 'Ca. Accumulibacter' and nitrate-reducing bacteria.
采用厌氧-缺氧序批式反应器（A2SBR），在一个将上流式厌氧污泥床与下流式悬挂海绵反应器相结合的节能污水处理系统中实现氮磷去除，以处理城市污水。污泥驯化后，A2SBR表现出令人满意的反硝化和除磷性能，出水总磷酸盐和硝酸盐浓度分别为8.4±3.4 mg-N L⁻¹和0.9±0.6 mg-P L⁻¹。16S rRNA基因序列和荧光原位杂交分析表明，“聚磷菌属未定种（Candidatus Accumulibacter phosphatis）”是主要的聚磷微生物。虽然未检测到聚磷菌的竞争菌“竞争聚磷菌未定种（Ca. Competibacter phosphatis）”，但脱氯单胞菌属（Dechloromonas spp.）数量丰富。ppk1基因序列分析表明，“聚磷菌属未定种（Ca. Accumulibacter）”的II型谱系占主导。结果表明，A2SBR中的反硝化和除磷可通过“聚磷菌属未定种（Ca. Accumulibacter）”与硝酸盐还原菌的协同作用实现。
