Wang J, Wang L M, Du W L, Chen L G, Liu S H, Wu J, Zhao J X, Yang Q H, Chen X H
Genetika. 2014 Jun;50(6):692-9. doi: 10.7868/s0016675814060137.
In previous studies, we developed a wheat-Psathyrostachys huashanica Keng disomic addition line 3-8-10-2, which exhibited high stripe rust resistance and could be used as a donor source for introducing novel disease resistance gene(s) into wheat in future breeding programs. It was identified using cytology, genomic in situ hybridization (GISH), EST-SSR, EST-STS and morphological analyses. However, these techniques are not suitable for breeding programs that require the rapid screening of large numbers of genotypes because they are highly technical and time-consuming. In this study, three Ns genome-specific SCAR markers were developed via random amplified polymorphic DNA (RAPD) markers. These SCAR markers were further validated using a complete set of wheat-P. huashanica disomic addition lines, which segregated the 5Ns disomic addition line individuals. Our results indicated that the SCAR markers associated with the 5Ns chromosome of P. huashanica and they provide a low cost, high efficiency, alternative tool for screening 5Ns chromosomes in a wheat background. These newly developed SCAR markers that species-specificity of the markers was proved by analysis of a wide range of cereal species, and specific for 5Ns chromosome, which should be useful in marker-assisted selection for wheat breeders who want to screen genotypes that may contain 5Ns chromatin.
在先前的研究中，我们培育出了一个小麦-华山新麦草二体附加系3-8-10-2，该附加系表现出高抗条锈病特性，可作为未来育种计划中向小麦导入新抗病基因的供体材料。我们通过细胞学、基因组原位杂交（GISH）、EST-SSR、EST-STS和形态学分析对其进行了鉴定。然而，这些技术不适用于需要快速筛选大量基因型的育种计划，因为它们技术要求高且耗时。在本研究中，通过随机扩增多态性DNA（RAPD）标记开发了三个Ns基因组特异性SCAR标记。利用一套完整的小麦-华山新麦草二体附加系对这些SCAR标记进行了进一步验证，该附加系对5Ns二体附加系个体进行了分离。我们的结果表明，这些SCAR标记与华山新麦草的5Ns染色体相关，它们为在小麦背景中筛选5Ns染色体提供了一种低成本、高效率的替代工具。通过对多种禾谷类物种的分析证明了这些新开发的SCAR标记具有物种特异性，且对5Ns染色体具有特异性，这对于想要筛选可能含有5Ns染色质的基因型的小麦育种者进行标记辅助选择应该是有用的。
