Kalyzhnaya O V, Itskovich V B
Genetika. 2014 Jul;50(7):765-76.
The diversity of bacteria associated with deep-water sponge Baikalospongia intermedia was evaluated by sequence analysis of 16S rRNA genes from two sponge samples collected in Lake Baikal from depths of 550 and 1204 m. A total of 64 operational taxonomic units, belonging to nine bacterial phyla, Proteobacteria (classes Alphaproteobacteria,. Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria), Actinobacteria, Planctomycetes, Cloroflexi, Verrucomicrobia, Acidobacteria, Chlorobi, and Nitrospirae, including candidate phylum WS5, were identified. Phylogenetic analysis showed that the examined communities contained phylotypes exhibiting homology to uncultured bacteria from different lake ecosystems, freshwater sediments, soil and geological formations. Moreover, a number of phylotypes were relative to psychrophilic, methane-oxidizing, sulfate-reducing bacteria, and to microorganisms resistant to the influence of heavy metals. It seems likely that the unusual habitation conditions of deep-water sponges contribute to the taxonomic diversity of associated bacteria and have an influence on the presence of functionally important microorganisms in bacterial communities.
通过对从贝加尔湖550米和1204米深处采集的两个海绵样本的16S rRNA基因进行序列分析，评估了与深水海绵中间贝加尔海绵相关的细菌多样性。共鉴定出64个操作分类单元，分属于9个细菌门，即变形菌门（包括α-变形菌纲、β-变形菌纲、γ-变形菌纲和δ-变形菌纲）、放线菌门、浮霉菌门、绿弯菌门、疣微菌门、酸杆菌门、绿菌门和硝化螺旋菌门，其中包括候选门WS5。系统发育分析表明，所检测的群落包含与来自不同湖泊生态系统、淡水沉积物、土壤和地质构造的未培养细菌具有同源性的系统发育型。此外，一些系统发育型与嗜冷菌、甲烷氧化菌、硫酸盐还原菌以及耐重金属影响的微生物相关。深水海绵不同寻常的栖息条件似乎有助于相关细菌的分类多样性，并对细菌群落中功能重要的微生物的存在产生影响。
