Romanenko Maria N, Shikov Anton E, Savina Iuliia A, Shmatov Fedor M, Nizhnikov Anton A, Antonets Kirill S
All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia.
Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.
Microorganisms. 2024 Nov 28;12(12):2450. doi: 10.3390/microorganisms12122450.
The dispersal of plant pathogens is a threat to the global economy and food industry which necessitates the need to discover efficient biocontrol agents such as bacteria, fungi, etc., inhibiting them. Here, we describe the strain b12.3 isolated from the soil of Olkhon Island in Lake Baikal, Russia. By applying the co-cultivation technique, we found that the strain inhibits the growth of plant pathogens, such as the bacteria , , and as well as the fungus . To elucidate the genomic fundament explaining these activities, we leveraged next-generation whole-genome sequencing and obtained a high-quality assembly based on short reads. The isolate bore seven known BGCs (biosynthetic gene clusters), including those responsible for producing bacillibactin, fengycin, and petrobactin. Moreover, the genome contained insecticidal genes encoding for App4Aa1, Tpp78Ba1, and Spp1Aa1 toxins, thus implicating possible pesticidal potential. We compared the genome with the 50 closest assemblies and found that b12.3 is enriched with BGCs. The genomic analysis also revealed that genomic architecture corresponds to the experimentally observed activity spectrum implying that the combination of produced secondary metabolites delineates the range of inhibited phytopathogens Therefore, this study deepens our knowledge of the biology and ecology of residing in the Lake Baikal region.
植物病原体的传播对全球经济和食品工业构成威胁，这使得有必要发现诸如细菌、真菌等能够抑制它们的高效生物防治剂。在此，我们描述了从俄罗斯贝加尔湖奥尔洪岛土壤中分离出的b12.3菌株。通过应用共培养技术，我们发现该菌株能够抑制植物病原体的生长，如细菌、 、 和 以及真菌 。为了阐明解释这些活性的基因组基础，我们利用了下一代全基因组测序技术，并基于短读长获得了高质量的组装结果。该分离株含有七个已知的生物合成基因簇（BGCs），包括负责产生杆菌肽、丰原素和石油菌素的基因簇。此外，该基因组还包含编码App4Aa1、Tpp78Ba1和Spp1Aa1毒素的杀虫基因，因此暗示了其可能的杀虫潜力。我们将该基因组与50个最相近的组装基因组进行了比较，发现b12.3富含生物合成基因簇。基因组分析还表明，基因组结构与实验观察到的活性谱相对应，这意味着所产生的次生代谢产物的组合划定了被抑制的植物病原体的范围。因此，本研究加深了我们对贝加尔湖地区 生物学和生态学的了解。
