Ge Zhijiang, Mao Shuhong, Li Yanqing, Liu Xiaoguang, Lu Fuping
Sheng Wu Gong Cheng Xue Bao. 2014 Sep;30(9):1481-5.
In order to discover the steroid biotransformation ability of filamentous fungus Aspergillus niger TCCC41650, we studied the fermentation of 4-androstene-3,17-dione with A. niger TCCC41650. The transformation product was purified, crystallized and determined as 16β-hydroxy-androst-4-ene-3,17-dione by X-ray single crystal diffraction method. The best fermentation condition was found to be pH 6.0, ethanol amount 2% with a substrate concentration of 1 per thousand, the transformation rate is 85.81% after 72 h. Based on the best of our knowledge, 16β-hydroxylation rarely occurs in microbial transformations of steroid. This study laid the foundation for the research of 16β-hydroxylation steroids
为了探究丝状真菌黑曲霉TCCC41650的甾体生物转化能力，我们研究了黑曲霉TCCC41650对4-雄烯-3,17-二酮的发酵过程。对转化产物进行了纯化、结晶，并通过X射线单晶衍射法确定为16β-羟基-雄甾-4-烯-3,17-二酮。发现最佳发酵条件为pH 6.0，乙醇添加量2%，底物浓度为千分之一，72小时后转化率为85.81%。据我们所知，在甾体的微生物转化中很少发生16β-羟基化反应。本研究为16β-羟基化甾体的研究奠定了基础。
