Mcy蛋白在链脲佐菌素诱导的糖尿病大鼠中的抗高血脂及生化活性
Antihyperlipidemic and biochemical activities of Mcy protein in streptozotocin induced diabetic rats.
作者信息
Marella Saritha, Maddirela Dilip Rajasekhar, Badri Kameswara Rao, Jyothi Kumar Malaka Venkateshwarulu, Chippada Apparao
机构信息
Department of Biochemistry, Sri Venkateswara University, Tirupati, AP, India.
出版信息
Cell Physiol Biochem. 2015;35(4):1326-34. doi: 10.1159/000373954. Epub 2015 Feb 12.
BACKGROUND
This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic "Mcy protein" isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model.
MATERIALS AND METHODS
Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests.
RESULTS
Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats.
CONCLUSION
Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.
背景
本研究旨在评估从刺角瓜果实中分离出的新型抗高血糖“Mcy蛋白”对链脲佐菌素诱导的糖尿病大鼠模型的保护作用。
材料与方法
野生型和链脲佐菌素诱导的糖尿病雄性Wistar白化大鼠，每天腹腔注射2.5毫克Mcy蛋白/千克体重或醋酸缓冲液，持续30天。测量空腹血糖、血清和组织脂质水平，并进行肝功能和肾功能测试的生化分析。
结果
与糖尿病对照组相比，Mcy蛋白显著降低了空腹血糖、血清和组织脂质水平（p<0.05），同时使治疗的糖尿病大鼠的肝功能和肾功能标志物水平恢复正常。我们的研究还显示，Mcy治疗的大鼠出现胰岛再生。
结论
Mcy蛋白可减轻高脂血症，并通过刺激胰岛素分泌帮助控制糖尿病，对肝脏和肾脏无明显毒性作用。
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