Kuo Hsi-Kung, Chen Yi-Hao, Wu Pei-Chang, Kuo Yu-Hsia
Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, ROC.
Ophthalmologica. 2015;233(3-4):198-203. doi: 10.1159/000371901. Epub 2015 Feb 17.
To investigate a new sustained-release formulation of dexamethasone (Ozurdex®) for inhibiting proliferative vitreoretinopathy (PVR) and its effect on the expression of retinal glial reaction and inflammation in experimental PVR eyes.
We used 30 pigmented rabbits for this study. One week after gas compression, the eyes were injected with 5 × 10(4) retinal pigment epithelial cells into the vitreous cavity to induce PVR. Concurrently, one eye also received an intravitreal injection of Ozurdex; the other eye was used as a control. PVR was graded by indirect ophthalmoscopy on days 1, 3, 7, 14, 21, and 28. The expression of the retinal glial reaction and inflammation in experimental PVR eyes were evaluated by Western blot analysis.
PVR severity increased gradually and peaked after 14 days, and no differences in PVR severity between the study and control groups were observed at any time point. The expression of glial fibrillary acid protein (GFAP) increased on days 7 and 14 in both the PVR control and study groups. While the use of Ozurdex in the study group showed less GFAP expression, this difference was not significant. The expression of tumor necrosis factor (TNF)-α and interleukin (IL)-6 significantly increased on days 7 and 14 in PVR control eyes. There was a significant difference in TNF-α between PVR control eyes and Ozurdex-treated eyes on days 7 (p < 0.001) and 14 (p = 0.019). Ozurdex in the study group showed lower IL-6 expression; however, this difference was not significant on days 7 (p = 0.063) and 14 (p = 0.052).
The intravitreal injection of Ozurdex suppressed the expression of inflammatory markers; however, it did not mitigate the severity of experimental PVR in this animal model. © 2015 S. Karger AG, Basel.
研究地塞米松新的缓释制剂（Ozurdex®）对增殖性玻璃体视网膜病变（PVR）的抑制作用及其对实验性PVR兔眼视网膜胶质反应和炎症表达的影响。
本研究使用30只有色家兔。气体压缩1周后，向玻璃体腔注射5×10(4)个视网膜色素上皮细胞以诱导PVR。同时，一只眼玻璃体内注射Ozurdex；另一只眼作为对照。在第1、3、7、14、21和28天通过间接检眼镜对PVR进行分级。通过蛋白质印迹分析评估实验性PVR兔眼中视网膜胶质反应和炎症的表达。
PVR严重程度逐渐增加并在14天后达到峰值，在任何时间点研究组和对照组之间的PVR严重程度均未观察到差异。PVR对照组和研究组中胶质纤维酸性蛋白（GFAP）的表达在第7天和第14天均增加。虽然研究组使用Ozurdex后GFAP表达较低，但这种差异不显著。肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的表达在PVR对照眼中第7天和第14天显著增加。在第7天（p<0.001）和第14天（p=0.019），PVR对照眼和Ozurdex治疗眼之间的TNF-α存在显著差异。研究组中的Ozurdex显示IL-6表达较低；然而，在第7天（p=0.063）和第14天（p=0.052）这种差异不显著。
玻璃体内注射Ozurdex可抑制炎症标志物的表达；然而，在该动物模型中它并未减轻实验性PVR的严重程度。©2015 S. Karger AG，巴塞尔。
